Academic Journal
Enhanced cell viscosity: a new phenotype associated with lamin A/C alterations
| العنوان: | Enhanced cell viscosity: a new phenotype associated with lamin A/C alterations |
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| المؤلفون: | Jebane, Cécile, Varlet, Alice-Anaïs, Karnat, Marc, Hernandez- Cedillo, Lucero, Lecchi, Amélie, Bedu, Frédéric, Desgrouas, Camille, Vigouroux, Corinne, Vantyghem, Marie-Christine, Viallat, Annie, Rupprecht, Jean-François, Helfer, Emmanuèle, Badens, Catherine |
| المساهمون: | Centre Interdisciplinaire de Nanoscience de Marseille (CINaM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Turing Centre for Living Systems Marseille (TCLS), Marseille medical genetics - Centre de génétique médicale de Marseille (MMG), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Physique Théorique - UMR 7332 (CPT), Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), CHU Saint-Antoine AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Recherche translationnelle sur le diabète - U 1190 (RTD), Institut Pasteur de Lille, Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille), Département de Biochimie Hôpital de la Timone - APHM, Hôpital de la Timone CHU - APHM (TIMONE), Amidex A-M-AAP-ID-17-66-170301-11.30, ANR-16-CONV-0001,CENTURI,CenTuri : Centre Turing des Systèmes vivants(2016) |
| المصدر: | EISSN: 2589-0042 ; iScience ; https://hal.science/hal-04205177 ; iScience, 2023, 26 (10), pp.107714. ⟨10.1016/j.isci.2023.107714⟩ |
| بيانات النشر: | CCSD Elsevier |
| سنة النشر: | 2023 |
| المجموعة: | Université de Toulon: HAL |
| مصطلحات موضوعية: | Lamin A/C, Lipodystrophy, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [PHYS]Physics [physics], [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] |
| الوصف: | International audience ; Lamin A/C is a well-established key contributor to nuclear stiffness and its role in nucleus mechanical properties has been extensively studied. However, its impact on whole cell mechanics has been poorly addressed, even less so in terms of measurable physical parameters. In the present study, microfluidic experiments combined with theoretical analyses were performed to provide a quantitative estimation of the whole cell mechanical properties. This allowed the characterization of mechanical cell changes induced by lamin A/C alterations resulting from Atazanavir treatment or lipodystrophy-associated LMNA R482W pathogenic variant. Results unveil an increase in the long-time viscosity as a signature of cells affected by lamin A/C alterations. In addition, they show that the whole cell response to mechanical stress is driven not only by the nucleus but also by the nucleo-cytoskeleton links and the microtubule network. This enhanced cell viscosity assessed by our microfluidic device could represent a useful diagnosis marker for lamin-related diseases. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/j.isci.2023.107714 |
| الاتاحة: | https://hal.science/hal-04205177 https://hal.science/hal-04205177v1/document https://hal.science/hal-04205177v1/file/iScience2023_Jebane%26al.pdf https://doi.org/10.1016/j.isci.2023.107714 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.54751561 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.isci.2023.107714 |
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