Academic Journal
Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog ; Upotreba mikrosatelita u proceni genetičkog diverziteta, kontroli roditeljstva i individualnoj identifikaciji pasarase kangal
| العنوان: | Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog ; Upotreba mikrosatelita u proceni genetičkog diverziteta, kontroli roditeljstva i individualnoj identifikaciji pasarase kangal |
|---|---|
| المؤلفون: | Dimitrijević, Vladimir, Ristanić, Marko, Stanišić, Ljubodrag, Drobnjak, Darko, Urošević, Milivoje, Ozkanal, Umit, Stanimirović, Zoran |
| المصدر: | Acta Veterinaria-Beograd |
| بيانات النشر: | Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | allele frequency, canine microsatellites, genetic characterization, Kangal, parentage testing |
| الوصف: | The Kangal Shepherd Dog is considered the most common dog breed of Turkish origin. This study investigated variations in ten autosomal microsatellite markers (PEZ01, PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 and FHC2079) for the purposes of genetic diversity assessment of the Kangal breed. In addition, the use of markers was assessed in parentage testing and individual identification within the Kangal breed. The microsatellite markers were typed in 51 Kangal dogs. The total number of alleles in the study population was 69. The mean number of alleles per locus was 6.9, and varied from four (FHC2079) to 12 (PEZ12). The polymorphic information content (PIC) ranged from 0.52 (FHC2079) to 0.87 (PEZ12), with the mean value for all loci of 0.717. Power of exclusion (PE) in 10 microsatellites investigated varied between 0.143 (FHC2054) and 0.472 (PEZ08) per locus. In order to determine the efficiency of using microsatellites for individual identification in the Kangal breed, power of discrimination (PD) and matching probability (MP) were calculated for each microsatellite marker. The panel achieved high combined MP (6.77 × 10-10) and high combined PD value of 99.99999%. The obtained results may contribute to further recognition of the Kangal breed, and confirm that the investigated microsatellites enable a reliable parentage testing and individual identification of the breed. ; Kangal rasa pasa se smatra jednom od najčešćih rasa turskog porekla. Ova studija je istraživala varijacije deset autozomalnih mikrosatelitskih markera (PEZ01, PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 i FHC2079) u svrhu određivanja genetičkog diverziteta pasa rase kangal. Pored toga, izvršena je procena mogućnosti primene ovih markera u individualnoj identifikaciji i u kontroli roditeljstva pasa rase kangal. Izvršena je tipizacija navedenih mikrosatelitskih markera kod 51 jedinke kangal rase. Ukupan broj alela u ispitivanoj populaciji iznosio je 69. Prosečan broj alela po lokusu iznosio je 6,9 sa varijacijama od ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//; 000540849900002 |
| DOI: | 10.2478/acve-2020-0012 |
| الاتاحة: | https://vet-erinar.vet.bg.ac.rs/handle/123456789/2039 https://doi.org/10.2478/acve-2020-0012 https://vet-erinar.vet.bg.ac.rs/bitstream/id/5567/Use_of_microsatellites_pub_2020.pdf |
| Rights: | openAccess ; https://creativecommons.org/licenses/by-nc-nd/4.0/ ; BY-NC-ND |
| رقم الانضمام: | edsbas.53E84D8F |
| قاعدة البيانات: | BASE |
| DOI: | 10.2478/acve-2020-0012 |
|---|