Academic Journal
Activation of Liver X Receptors and Peroxisome Proliferator-Activated Receptors by Lipid Extracts of Brown Seaweeds:A Potential Application in Alzheimer's Disease?
| العنوان: | Activation of Liver X Receptors and Peroxisome Proliferator-Activated Receptors by Lipid Extracts of Brown Seaweeds:A Potential Application in Alzheimer's Disease? |
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| المؤلفون: | Martens, Nikita, Zhan, Na, Voortman, Gardi, Leijten, Frank P J, van Rheenen, Connor, van Leerdam, Suzanne, Geng, Xicheng, Huybrechts, Michiel, Liu, Hongbing, Jonker, Johan W, Kuipers, Folkert, Lütjohann, Dieter, Vanmierlo, Tim, Mulder, Monique T |
| المصدر: | Martens , N , Zhan , N , Voortman , G , Leijten , F P J , van Rheenen , C , van Leerdam , S , Geng , X , Huybrechts , M , Liu , H , Jonker , J W , Kuipers , F , Lütjohann , D , Vanmierlo , T & Mulder , M T 2023 , ' Activation of Liver X Receptors and Peroxisome Proliferator-Activated Receptors by Lipid Extracts of Brown Seaweeds : A Potential Application in Alzheimer's Disease? ' , Nutrients , vol. 15 , no. 13 , 3004 . https://doi.org/10.3390/nu15133004 |
| سنة النشر: | 2023 |
| المجموعة: | University of Groningen research database |
| مصطلحات موضوعية: | Mice, Animals, Liver X Receptors/genetics, Alzheimer Disease/drug therapy, PPAR alpha/genetics, Tandem Mass Spectrometry, Receptors, Cytoplasmic and Nuclear/genetics, Cholesterol/metabolism, Seaweed, Fatty Acids/metabolism |
| الوصف: | The nuclear liver X receptors (LXRα/β) and peroxisome proliferator-activated receptors (PPARα/γ) are involved in the regulation of multiple biological processes, including lipid metabolism and inflammation. The activation of these receptors has been found to have neuroprotective effects, making them interesting therapeutic targets for neurodegenerative disorders such as Alzheimer's Disease (AD). The Asian brown seaweed Sargassum fusiforme contains both LXR-activating (oxy)phytosterols and PPAR-activating fatty acids. We have previously shown that dietary supplementation with lipid extracts of Sargassum fusiforme prevents disease progression in a mouse model of AD, without inducing adverse effects associated with synthetic pan-LXR agonists. We now determined the LXRα/β- and PPARα/γ-activating capacity of lipid extracts of six European brown seaweed species ( Alaria esculenta, Ascophyllum nodosum, Fucus vesiculosus, Himanthalia elongata, Saccharina latissima, and Sargassum muticum) and the Asian seaweed Sargassum fusiforme using a dual luciferase reporter assay. We analyzed the sterol and fatty acid profiles of the extracts by GC-MS and UPLC MS/MS, respectively, and determined their effects on the expression of LXR and PPAR target genes in several cell lines using quantitative PCR. All extracts were found to activate LXRs, with the Himanthalia elongata extract showing the most pronounced efficacy, comparable to Sargassum fusiforme, for LXR activation and transcriptional regulation of LXR-target genes. Extracts of Alaria esculenta, Fucus vesiculosus, and Saccharina latissima showed the highest capacity to activate PPARα, while extracts of Alaria esculenta, Ascophyllum nodosum, Fucus vesiculosus, and Sargassum muticum showed the highest capacity to activate PPARγ, comparable to Sargassum fusiforme extract. In CCF-STTG1 astrocytoma cells, all extracts induced expression of cholesterol efflux genes ( ABCG1, ABCA1, and APOE) and suppressed expression of cholesterol and fatty acid synthesis genes ( DHCR7, DHCR24, HMGCR ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.3390/nu15133004 |
| الاتاحة: | https://hdl.handle.net/11370/80d764ba-569d-4b21-8139-1f32da3ac116 https://research.rug.nl/en/publications/80d764ba-569d-4b21-8139-1f32da3ac116 https://doi.org/10.3390/nu15133004 https://pure.rug.nl/ws/files/744469650/nutrients-15-03004-v3.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.53A9B3FE |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/nu15133004 |
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