Academic Journal
Ragweed plants grown under elevated CO 2 levels produce pollen which elicit stronger allergic lung inflammation.
| العنوان: | Ragweed plants grown under elevated CO 2 levels produce pollen which elicit stronger allergic lung inflammation. |
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| المؤلفون: | Rauer, D., Gilles, S., Wimmer, M., Frank, U., Müller, C., Musiol, S., Vafadari, B., Aglas, L., Ferreira, F., Schmitt-Kopplin, P., Durner, J., Winkler, J.B., Ernst, D., Behrendt, H., Schmidt-Weber, C.B., Traidl-Hoffmann, C., Alessandrini, F. |
| المصدر: | Allergy 76, 1718-1730 (2021) |
| بيانات النشر: | Wiley |
| سنة النشر: | 2021 |
| المجموعة: | PuSH - Publikationsserver des Helmholtz Zentrums München |
| مصطلحات موضوعية: | Allergic Lung Inflammation, Carbon Dioxide, Climate Change, Pollen Metabolome, Ragweed |
| الوصف: | Background Common ragweed has been spreading as a neophyte in Europe. Elevated CO2 levels, a hallmark of global climate change, have been shown to increase ragweed pollen production, but their effects on pollen allergenicity remain to be elucidated.Methods Ragweed was grown in climate-controlled chambers under normal (380 ppm, control) or elevated (700 ppm, based on RCP4.5 scenario) CO2 levels. Aqueous pollen extracts (RWE) from control- or CO2-pollen were administered in vivo in a mouse model for allergic disease (daily for 3-11 days, n = 5) and employed in human in vitro systems of nasal epithelial cells (HNECs), monocyte-derived dendritic cells (DCs), and HNEC-DC co-cultures. Additionally, adjuvant factors and metabolites in control- and CO2-RWE were investigated using ELISA and untargeted metabolomics.Results In vivo, CO2-RWE induced stronger allergic lung inflammation compared to control-RWE, as indicated by lung inflammatory cell infiltrate and mediators, mucus hypersecretion, and serum total IgE. In vitro, HNECs stimulated with RWE increased indistinctively the production of pro-inflammatory cytokines (IL-8, IL-1 beta, and IL-6). In contrast, supernatants from CO2-RWE-stimulated HNECs, compared to control-RWE-stimulated HNECS, significantly increased TNF and decreased IL-10 production in DCs. Comparable results were obtained by stimulating DCs directly with RWEs. The metabolome analysis revealed differential expression of secondary plant metabolites in control- vs CO2-RWE. Mixes of these metabolites elicited similar responses in DCs as compared to respective RWEs.Conclusion Our results indicate that elevated ambient CO2 levels elicit a stronger RWE-induced allergic response in vivo and in vitro and that RWE increased allergenicity depends on the interplay of multiple metabolites. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0105-4538 1398-9995 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/33037672; info:eu-repo/semantics/altIdentifier/wos/WOS:000584139400001; info:eu-repo/semantics/altIdentifier/isbn/0105-4538; info:eu-repo/semantics/altIdent; https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=60561; urn:isbn:0105-4538; urn:issn:0105-4538; urn:issn:1398-9995 |
| DOI: | 10.1111/all.14618 |
| الاتاحة: | https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=60561 https://doi.org/10.1111/all.14618 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.537B9668 |
| قاعدة البيانات: | BASE |
| تدمد: | 01054538 13989995 |
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| DOI: | 10.1111/all.14618 |