Academic Journal
A Potent Recombinant Polyclonal Antibody Therapeutic for Protection Against New Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern
| العنوان: | A Potent Recombinant Polyclonal Antibody Therapeutic for Protection Against New Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern |
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| المؤلفون: | Wayham, Nicholas P, Niedecken, Ariel R, Simons, Jan Fredrik, Chiang, Yao Y, Medina-Cucurella, Angélica V, Mizrahi, Rena A, Wagner, Ellen K, Gras, Ashley, Segal, Ilana, Witte, Peyton, Enstrom, Alexis, Bountouvas, Aristea, Nelson, Sabrina M, Weinberger, Tess, Tan, David, Asensio, Michael A, Subramanian, Alagu, Lim, Yoong Wearn, Adler, Adam S, Keating, Sheila M |
| المساهمون: | GigaGen, Grifols |
| المصدر: | The Journal of Infectious Diseases ; volume 228, issue 5, page 555-563 ; ISSN 0022-1899 1537-6613 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2023 |
| الوصف: | Emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) possess mutations that prevent antibody therapeutics from maintaining antiviral binding and neutralizing efficacy. Monoclonal antibodies (mAbs) shown to neutralize Wuhan-Hu-1 SARS-CoV-2 (ancestral) strain have reduced potency against newer variants. Plasma-derived polyclonal hyperimmune drugs have improved neutralization breadth compared with mAbs, but lower titers against SARS-CoV-2 require higher dosages for treatment. We previously developed a highly diverse, recombinant polyclonal antibody therapeutic anti-SARS-CoV-2 immunoglobulin hyperimmune (rCIG). rCIG was compared with plasma-derived or mAb standards and showed improved neutralization of SARS-CoV-2 across World Health Organization variants; however, its potency was reduced against some variants relative to ancestral, particularly omicron. Omicron-specific antibody sequences were enriched from yeast expressing rCIG-scFv and exhibited increased binding and neutralization to omicron BA.2 while maintaining ancestral strain binding and neutralization. Polyclonal antibody libraries such as rCIG can be utilized to develop antibody therapeutics against present and future SARS-CoV-2 threats. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/infdis/jiad102 |
| DOI: | 10.1093/infdis/jiad102/50188641/jiad102.pdf |
| الاتاحة: | http://dx.doi.org/10.1093/infdis/jiad102 https://academic.oup.com/jid/advance-article-pdf/doi/10.1093/infdis/jiad102/50188641/jiad102.pdf https://academic.oup.com/jid/article-pdf/228/5/555/51312804/jiad102.pdf |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.5364CFF1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/infdis/jiad102 |
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