Academic Journal
Fecal microbiota profiles in treatment-naive pediatric inflammatory bowel disease - associations with disease phenotype, treatment, and outcome
| العنوان: | Fecal microbiota profiles in treatment-naive pediatric inflammatory bowel disease - associations with disease phenotype, treatment, and outcome |
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| المؤلفون: | Olbjern, C., Smastuen, M.C., Thiis-Evensen, E., Nakstad, B., Vatn, M.H., Jahnsen, J., Ricanek, P., Vatn, S., Moen, A.E.F., Tannaes, T.M., Lindstrom, J.C., Soderholm, J.D., Halfvarson, J., Gomollon, F., Casen, C., Karlsson, M.K., Kalla, R., Adams, A.T., Satsangi, J., Perminow, G. |
| سنة النشر: | 2019 |
| المجموعة: | Digital Repository of University of Zaragoza (ZAGUAN) |
| الوصف: | Purpose: Imbalance in the microbiota, dysbiosis, has been identified in inflammatory bowel disease (IBD). We explored the fecal microbiota in pediatric patients with treatment-naive IBD, non-IBD patients with gastrointestinal symptoms and healthy children, its relation to IBD subgroups, and treatment outcomes. Patients and methods: Fecal samples were collected from 235 children below 18 years of age. Eighty children had Crohn''s disease (CD), 27 ulcerative colitis (UC), 3 IBD unclassified, 50 were non-IBD symptomatic patients, and 75 were healthy. The bacterial abundance of 54 predefined DNA markers was measured with a 16S rRNA DNA-based test using GA-Map (TM) technology at diagnosis and after therapy in IBD patients. Results: Bacterial abundance was similarly reduced in IBD and non-IBD patients in 51 of 54 markers compared to healthy patients (P<0.001). Only Prevotella was more abundant in patients (P<0.01). IBD patients with ileocolitis or total colitis had more Ruminococcus gnavus (P=0.02) than patients with colonic CD or left-sided UC. CD patients with upper gastrointestinal manifestations had higher Veillonella abundance (P<0.01). IBD patients (58%) who received biologic therapy had lower baseline Firmicutes and Mycoplasma hominis abundance (P<0.01) than conventionally treated. High Proteobacteria abundance was associated with stricturing/penetrating CD, surgery (P<0.01), and nonmucosal healing (P<0.03). Low Faecalibacterium prausnitzii abundance was associated with prior antibiotic therapy (P=0.001), surgery (P=0.02), and nonmucosal healing (P<0.03). After therapy, IBD patients had unchanged dysbiosis. Conclusion: Fecal microbiota profiles differentiated IBD and non-IBD symptomatic children from healthy children, but displayed similar dysbiosis in IBD and non-IBD symptomatic patients. Pretreatment fecal microbiota profiles may be of prognostic value and aid in treatment individualization in pediatric IBD as severe dysbiosis was associated with an extensive, complicated phenotype, ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | info:eu-repo/grantAgreement/EC/FP7/305676/EU/Inflammatory Bowel Disease CHARACTERization by a multi-modal integrated biomarker study/IBD-CHARACTER; http://zaguan.unizar.es/record/78221 |
| DOI: | 10.2147/CEG.S186235 |
| الاتاحة: | http://zaguan.unizar.es/record/78221 https://doi.org/10.2147/CEG.S186235 |
| Rights: | by-nc ; http://creativecommons.org/licenses/by-nc/3.0/es/ |
| رقم الانضمام: | edsbas.533AC672 |
| قاعدة البيانات: | BASE |
| DOI: | 10.2147/CEG.S186235 |
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