Academic Journal
MicroRNA-200c restoration reveals a cytokine profile to enhance M1 macrophage polarization in breast cancer
| العنوان: | MicroRNA-200c restoration reveals a cytokine profile to enhance M1 macrophage polarization in breast cancer |
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| المؤلفون: | Williams, Michelle M., Christenson, Jessica L., O’Neill, Kathleen I., Hafeez, Sabrina A., Ihle, Claire L., Spoelstra, Nicole S., Slansky, Jill E., Richer, Jennifer K. |
| المساهمون: | U.S. Department of Defense, U.S. Department of Health & Human Services | NIH | National Cancer Institute, U.S. Department of Health & Human Services | National Institutes of Health |
| المصدر: | npj Breast Cancer ; volume 7, issue 1 ; ISSN 2374-4677 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2021 |
| الوصف: | Many immune suppressive mechanisms utilized by triple negative breast cancer (TNBC) are regulated by oncogenic epithelial-to-mesenchymal transition (EMT). How TNBC EMT impacts innate immune cells is not fully understood. To determine how TNBC suppresses antitumor macrophages, we used microRNA-200c (miR-200c), a powerful repressor of EMT, to drive mesenchymal-like mouse mammary carcinoma and human TNBC cells toward a more epithelial state. MiR-200c restoration significantly decreased growth of mouse mammary carcinoma Met-1 cells in culture and in vivo. Cytokine profiling of Met-1 and human BT549 cells revealed that miR-200c upregulated cytokines, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), promoted M1 antitumor macrophage polarization. Cytokines upregulated by miR-200c correlated with an epithelial gene signature and M1 macrophage polarization in BC patients and predicted a more favorable overall survival for TNBC patients. Our findings demonstrate that immunogenic cytokines (e.g., GM-CSF) are suppressed in aggressive TNBC, warranting further investigation of cytokine-based therapies to limit disease recurrence. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41523-021-00273-1 |
| الاتاحة: | http://dx.doi.org/10.1038/s41523-021-00273-1 https://www.nature.com/articles/s41523-021-00273-1.pdf https://www.nature.com/articles/s41523-021-00273-1 |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.52C29124 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41523-021-00273-1 |
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