Academic Journal
Pin1-mediated modification prolongs the nuclear retention of β-Catenin in Wnt3a-induced osteoblast differentiation
| العنوان: | Pin1-mediated modification prolongs the nuclear retention of β-Catenin in Wnt3a-induced osteoblast differentiation |
|---|---|
| المؤلفون: | H-R Shin, R Islam, W-J Yoon, T Lee, Y-D Cho, H-S Bae, B-S Kim, K-M Woo, J-H Baek, H-M Ryoo |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | β-catenin, cell differentiation, nuclear translocation, osteoblast, Wnt signaling, peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1), adenomatous polyposis coli (APC), osteogenesis, peptidyl prolyl cis-trans isomerization, 110399 Clinical Sciences not elsewhere classified, 3202 Clinical sciences |
| الوصف: | The canonical Wnt signaling pathway, in which β-catenin nuclear localization is a crucial step, plays an important role in osteoblast differentiation. Pin1, a prolyl isomerase, is also known as a key enzyme in osteogenesis. However, the role of Pin1 in canonical Wnt signal-induced osteoblast differentiation is poorly understood. We found that Pin1 deficiency caused osteopenia and reduction of β-catenin in bone lining cells. Similarly, Pin1 knockdown or treatment with Pin1 inhibitors strongly decreased the nuclear β-catenin level, TOP flash activity, and expression of bone marker genes induced by canonical Wnt activation and vice versa in Pin1 overexpression. Pin1 interacts directly with and isomerizes β-catenin in the nucleus. The isomerized β-catenin could not bind to nuclear adenomatous polyposis coli, which drives β-catenin out of the nucleus for proteasomal degradation, which consequently increases the retention of β-catenin in the nucleus and might explain the decrease of β-catenin ubiquitination. These results indicate that Pin1 could be a critical target to modulate β-catenin-mediated osteogenesis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | http://hdl.handle.net/10536/DRO/DU:30096251 |
| الاتاحة: | http://hdl.handle.net/10536/DRO/DU:30096251 https://figshare.com/articles/journal_contribution/Pin1-mediated_modification_prolongs_the_nuclear_retention_of_-Catenin_in_Wnt3a-induced_osteoblast_differentiation/20852851 |
| Rights: | All Rights Reserved |
| رقم الانضمام: | edsbas.52B648C |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |