Book
(Hyper)thermophilic Enzymes: Production and Purification
| العنوان: | (Hyper)thermophilic Enzymes: Production and Purification |
|---|---|
| المؤلفون: | Falcicchio, P., Levisson, M., Kengen, S.W.M., Koutsopoulos, S. |
| المصدر: | Protein Downstream Processing ; ISBN: 9781627039765 |
| بيانات النشر: | Humana Press |
| سنة النشر: | 2014 |
| المجموعة: | Wageningen UR (University & Research Centre): Digital Library |
| مصطلحات موضوعية: | Biocatalysis, Heterologous production, His-tag, Immobilized metal affinity chromatography (IMAC), Protein purification, Size exclusion chromatography (SEC), Thermal stability, Thermozymes |
| الوصف: | The discovery of thermophilic and hyperthermophilic microorganisms, thriving at environmental temperatures near or above 100 °C, has revolutionized our ideas about the upper temperature limit at which life can exist. The characterization of (hyper)thermostable proteins has broadened our understanding and presented new opportunities for solving one of the most challenging problems in biophysics: how is structural stability and biological function maintained at high temperatures where “normal” proteins undergo dramatic structural changes? In our laboratory we have purified and studied many thermostable and hyperthermostable proteins in an attempt to determine the molecular basis of heat stability. Here, we present methods to express such proteins and enzymes in E. coli and provide a general protocol for overproduction and purification. The ability to produce enzymes that retain their stability and activity at elevated temperatures creates exciting opportunities for a wide range of biocatalytic applications |
| نوع الوثيقة: | book part |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-1-62703-976-5 1-62703-976-7 |
| Relation: | https://edepot.wur.nl/310578 |
| DOI: | 10.1007/978-1-62703-977-2_34 |
| الاتاحة: | https://research.wur.nl/en/publications/hyperthermophilic-enzymes-production-and-purification https://doi.org/10.1007/978-1-62703-977-2_34 |
| Rights: | Wageningen University & Research |
| رقم الانضمام: | edsbas.52AEFE3D |
| قاعدة البيانات: | BASE |
| ردمك: | 9781627039765 1627039767 |
|---|---|
| DOI: | 10.1007/978-1-62703-977-2_34 |