Academic Journal
Therapeutic effect of fully human anti-Nrp-1 antibody on non-small cell lung cancer in vivo and in vitro.
| العنوان: | Therapeutic effect of fully human anti-Nrp-1 antibody on non-small cell lung cancer in vivo and in vitro. |
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| المؤلفون: | Zhang, Bo, Liu, Qin, Li, Lin, Ye, Yingchun, Guo, Xiyuan, Xu, Wenfeng, Chen, Ligang, Mo, Xianming, Nian, Siji, Yuan, Qing |
| المصدر: | Cancer Immunol Immunother ; ISSN:1432-0851 ; Volume:74 ; Issue:2 |
| بيانات النشر: | Springer |
| سنة النشر: | 2025 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | IgG1 antibody, NSCLC, Nrp-1, Tumor immunotherapy |
| الوصف: | Although immune checkpoint inhibitors have changed the treatment paradigm for non-small cell lung cancer (NSCLC), not all patients benefit from them. Therefore, there is an urgent need to explore novel immune checkpoint inhibitors. Neuropilin-1 (Nrp-1) is a unique immune checkpoint capable of exerting antitumor effects through CD8+ T cells. It is also a T-cell memory checkpoint that regulates long-term antitumor immunity. However, its role in NSCLC remains unclear. The aim of this study was to develop a fully human anti-Nrp-1 antibody with therapeutic effects against NSCLC in vitro and in vivo. We screened and constructed of a high-affinity anti-Nrp-1 IgG antibody from a constructed high-capacity fully human single-chain fragment variable (scFv) phage library. This novel anti-Nrp-1 IgG antibody partially restored the killing function of exhausted CD8+ T cells in malignant pleural fluid in vitro. Co-culture of peripheral blood mononuclear cells (PBMC) with A549 and the addition of anti-Nrp1-IgG enhanced the killing of A549 target cells, leading to an increase in late-stage apoptosis of target cells. Importantly, anti-Nrp1-IgG treatment significantly reduced tumor volume in a mouse model of lung cancer with humanized immune system. These findings suggest that 53-IgG has a promising application as a potent Nrp-1-targeting agent in NSCLC immunotherapy. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://doi.org/10.1007/s00262-024-03893-1; https://pubmed.ncbi.nlm.nih.gov/39751948 |
| DOI: | 10.1007/s00262-024-03893-1 |
| الاتاحة: | https://doi.org/10.1007/s00262-024-03893-1 https://pubmed.ncbi.nlm.nih.gov/39751948 |
| Rights: | © 2024. The Author(s). |
| رقم الانضمام: | edsbas.52398120 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00262-024-03893-1 |
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