Academic Journal
Parental sex-dependent effects of either maternal or paternal eNOS deficiency on the offspring’s phenotype without transmission of the parental eNOS deficiency to the offspring
| العنوان: | Parental sex-dependent effects of either maternal or paternal eNOS deficiency on the offspring’s phenotype without transmission of the parental eNOS deficiency to the offspring |
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| المؤلفون: | Zhang, Xiaoli, Reichetzeder, Christoph, Liu, Yvonne, Hocher, Johann-Georg, Hasan, Ahmed A., Lin, Ge, Kleuser, Burkhard, Hu, Liang, Hocher, Berthold |
| سنة النشر: | 2023 |
| المجموعة: | FU Berlin: Refubium |
| مصطلحات موضوعية: | maternal and paternal programming, eNOS, metabolomics, sex-dependent effects, offspring, ddc:570 |
| الوصف: | Background: Preclinical animal studies and clinical studies indicate that both maternal as well as paternal genetic alterations/gene defects might affect the phenotype of the next-generation without transmissions of the affected gene. Currently, the question of whether the same genetic defect present in the mother or father leads to a similar phenotype in the offspring remains insufficiently elucidated. Methods: In this head-to-head study, we crossbred female and male mice with heterozygous endothelial eNOS knockout (eNOS+/−) with male and female wild-type (wt) mice, respectively. Subsequently, we compared the phenotype of the resulting wt offspring with that of wt offspring born to parents with no eNOS deficiency. Results: Wt female offspring of mothers with heterozygous eNOS showed elevated liver fat accumulation, while wt male offspring of fathers with heterozygous eNOS exhibited increased fasting insulin, heightened insulin levels after a glucose load, and elevated liver glycogen content. By quantitative mass-spectrometry it was shown that concentrations of six serum metabolites (lysoPhosphatidylcholine acyl C20:3, phosphatidylcholine diacyl C36:2, phosphatidylcholine diacyl C38:1, phosphatidylcholine acyl-alkyl C34:1, phosphatidylcholine acyl-alkyl C36:3, and phosphatidylcholine acyl-alkyl C42:5 (PC ae C42:5) as well as four liver carbon metabolites (fructose 6-phosphate, fructose 1,6-bisphosphate, glucose 6-phosphate and fumarate) were different between wt offspring with eNOS+/− mothers and wt offspring with eNOS+/− fathers. Importantly, fumarate was inversely correlated with the liver fat accumulation in female offspring with eNOS+/− mothers and increased liver glycogen in offspring of both sexes with eNOS+/− fathers. The qRT-PCR results revealed that the gene expression patterns were different between wt offspring with eNOS+/− mothers and those offspring with eNOS+/− fathers. Different gene expression patterns were correlated with different observed phenotypic changes in male/female offspring born to ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 13 Seiten; application/pdf |
| اللغة: | English |
| Relation: | https://refubium.fu-berlin.de/handle/fub188/43284; http://dx.doi.org/10.17169/refubium-43000 |
| DOI: | 10.17169/refubium-43000 |
| DOI: | 10.3389/fphys.2023.1306178 |
| الاتاحة: | https://refubium.fu-berlin.de/handle/fub188/43284 https://doi.org/10.17169/refubium-43000 https://doi.org/10.3389/fphys.2023.1306178 |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.52243AB5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17169/refubium-43000 |
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