Academic Journal
Natural deep eutectic solvents protect RNA from thermal-induced degradation
| العنوان: | Natural deep eutectic solvents protect RNA from thermal-induced degradation |
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| المؤلفون: | Mattioli, Roberto, Maggiore, Anna, Di Risola, Daniel, Federico, Rodolfo, D'Erme, Maria, Francioso, Antonio, Mosca, Luciana |
| المساهمون: | Mattioli, Roberto, Maggiore, Anna, Di Risola, Daniel, Federico, Rodolfo, D'Erme, Maria, Francioso, Antonio, Mosca, Luciana |
| سنة النشر: | 2023 |
| المجموعة: | IRIS Universita degli Studi di Teramo |
| مصطلحات موضوعية: | DES, NaDES, RNA thermal degradation |
| الوصف: | RNA is a fundamental nucleic acid for life and it plays important roles in the regulation of gene transcription, post-transcriptional regulation, and epigenetic regulation. Recently, the focus on this nucleic acid has significantly increased due to the development of mRNA vaccines and RNA-based gene therapy protocols. Unfortunately, RNA based products show constrains mainly owing to instability and easy degradability of the RNA molecules. Indeed, unlike the DNA molecule which has a great intrinsic stability, RNA is more prone to degradation and this process is accelerated under thermal treatment. Here we describe a method that involves the use of Natural Deep Eutectic Solvents (NaDES) capable of slowing down RNA degradation process. Our re-sults show that this technology seems suitable for improving the stability of specific RNA molecules particularly susceptible to thermal-induced degradation. Therefore, this technique represents a valuable tool to stabilize RNA molecules used in gene therapy and mRNA vaccines. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/37549802; info:eu-repo/semantics/altIdentifier/wos/WOS:001060868700001; volume:745; firstpage:109714; journal:ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS; https://hdl.handle.net/11575/136840; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85166938679 |
| DOI: | 10.1016/j.abb.2023.109714 |
| الاتاحة: | https://hdl.handle.net/11575/136840 https://doi.org/10.1016/j.abb.2023.109714 |
| رقم الانضمام: | edsbas.51ADEFD5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.abb.2023.109714 |
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