The off-target effects of shGli2.

التفاصيل البيبلوغرافية
العنوان: The off-target effects of shGli2.
المؤلفون: Yu-Hao Chang (11099080), Hoi-Lam Tam (2054272), Meng-Chien Lu (11099083), Huei-Sheng Huang (132681)
سنة النشر: 2021
المجموعة: Smithsonian Institution: Digital Repository
مصطلحات موضوعية: Biochemistry, Microbiology, Cell Biology, Molecular Biology, Pharmacology, Immunology, Developmental Biology, Cancer, Science Policy, Hematology, Infectious Diseases, Plant Biology, Virology, Ser 9 residue, Gli 2 activity, GSK 3β kinase, urothelial carcinoma cells Patients, Gli 2 pathway, experience gemcitabine resistance, NGR, Flag-GSK S 9D, phospho-AKT ser 473, Flag-GSK S 9A, GLI, Gli 2, gemcitabine-resistant NTUB 1, phospho-GSK 3β ser 9, Gemcitabine-induced Gli-dependent a., NTUB 1 cells, benefit UC patients
الوصف: (A) Cells were co-transfected with reporters and pCS2MT-Gli2ΔN plasmids in T24 cells, and then harvested for the detection of their Gli2-luc activity. (B) To reduce the off-target effects, we co-transfected 4 specific shRNAs with different targets to Gli2 in NGR cells. The pLKO.1-shLuc vector was used as a vehicle control. (C) To perform rescue experiments, we co-transfected reporters, shGli2 , and pCS2MT-Gli2ΔN plasmids in NGR cells, and then harvested for the detection of their Gli2-luc activity. (TIF)
نوع الوثيقة: still image
اللغة: unknown
Relation: https://figshare.com/articles/figure/The_off-target_effects_of_shGli2_/14934289
DOI: 10.1371/journal.pone.0254011.s001
الاتاحة: https://doi.org/10.1371/journal.pone.0254011.s001
Rights: CC BY 4.0
رقم الانضمام: edsbas.513FCD8
قاعدة البيانات: BASE
الوصف
DOI:10.1371/journal.pone.0254011.s001