Academic Journal
Extracellular Vesicles from Cod (Gadus morhua L.) Mucus contain Innate Immune Factors and Deiminated Protein Cargo
| العنوان: | Extracellular Vesicles from Cod (Gadus morhua L.) Mucus contain Innate Immune Factors and Deiminated Protein Cargo |
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| المؤلفون: | Magnadóttir, B., Kraev, I., Gudmundsdottir, S., Dodds, A.W., Lange, S. |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2019 |
| المجموعة: | University of Westminster: WestminsterResearch |
| مصطلحات موضوعية: | extracellular vesicles (EVs), mucosal immunity, peptidylarginine deiminase (PAD), protein deimination, complement C3, CRP, cod (Gadus morhua L.) |
| الوصف: | Extracellular vesicles are released from cells and participate in cell communication via transfer of protein and genetic cargo derived from the parent cells. EVs play roles in normal physiology and immunity and are also linked to various pathological processes. Peptidylarginine deiminases (PADs) are phylogenetically conserved enzymes with physiological and pathophysiological roles. PADs cause post-translational protein deimination, resulting in structural and, in some cases, functional changes in target proteins and are also linked to EV biogenesis. This study describes for the first time EVs isolated from cod mucosa. Mucosal EVs were characterised by electron microscopy, nanoparticle tracking analysis and EV-specific surface markers. Cod mucosal EVs were found to carry PAD, complement component C3 and C-reactive proteins. C3 was found to be deiminated in both whole mucus and mucosal EVs, with some differences, and further 6 deiminated immune and cytoskeletal proteins were identified in EVs by LC-MS/MS analysis. As mucosal surfaces of teleost fish reflect human mucosal surfaces, these findings may provide useful insights into roles of EVs in mucosal immunity throughout phylogeny. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | https://westminsterresearch.westminster.ac.uk/download/ff97b4ecc5dcc254a6562834da0af9d0a810a2ac8824ab756d24828aad954032/1856970/1-s2.0-S0145305X19302010-main.pdf; https://doi.org/10.1016/j.dci.2019.103397; Magnadóttir, B., Kraev, I., Gudmundsdottir, S., Dodds, A.W. and Lange, S. 2019. Extracellular Vesicles from Cod (Gadus morhua L.) Mucus contain Innate Immune Factors and Deiminated Protein Cargo. Developmental and Comparative Immunology. p. 103397 103397. https://doi.org/10.1016/j.dci.2019.103397 |
| DOI: | 10.1016/j.dci.2019.103397 |
| الاتاحة: | https://westminsterresearch.westminster.ac.uk/item/qv349/extracellular-vesicles-from-cod-gadus-morhua-l-mucus-contain-innate-immune-factors-and-deiminated-protein-cargo https://westminsterresearch.westminster.ac.uk/download/ff97b4ecc5dcc254a6562834da0af9d0a810a2ac8824ab756d24828aad954032/1856970/1-s2.0-S0145305X19302010-main.pdf https://doi.org/10.1016/j.dci.2019.103397 |
| Rights: | CC BY-NC-ND 4.0 |
| رقم الانضمام: | edsbas.50FF2616 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.dci.2019.103397 |
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