Academic Journal
Cryopreservation of the mangaba tree ( Hancornia speciosa Gomes): a protocol for long-term storage
| العنوان: | Cryopreservation of the mangaba tree ( Hancornia speciosa Gomes): a protocol for long-term storage |
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| المؤلفون: | Paulo Augusto Almeida Santos, Renato Paiva, Luciano Coutinho Silva, Ana Cristina de Souza, Marlucia Cruz de Santana, Diogo Pedrosa Corrêa da Silva |
| المصدر: | Acta Scientiarum: Agronomy, Vol 37, Iss 3, Pp 289-296 (2015) |
| بيانات النشر: | Eduem (Editora da Universidade Estadual de Maringá) |
| سنة النشر: | 2015 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | native species, in vitro conservation, recalcitrant seeds, plant genetic diversity conservation, vitrification, droplet vitrification, Agriculture (General), S1-972 |
| الوصف: | The aim of this study was to evaluate the efficiency of vitrification and droplet vitrification for the cryopreservation of Hancornia speciosa shoot tips. The shoot tips were subjected to four different periods of exposure (15, 30, 45 and 60 min.) to plant vitrification solution 2 (PVS2) before plunging into liquid nitrogen. We evaluated the regrowth of H. speciosa shoot tips that were cryopreserved by the classical vitrification technique and by the droplet vitrification technique. Shoot tips were submitted to different periods of pre-culture (absence, 24 or 48h) in a medium containing 0.3 M sucrose prior to cryopreservation. With a PVS2 exposure period of 60 min., significant differences were observed between the two techniques used in this study with respect to regrowth rates; however, both cryopreservation methods allowed for a shoot tip regrowth rate of over 70%. Use of a preculture step increased the regrowth rate of cryopreserved shoot tips. Shoot tip cryopreservation techniques employing vitrification and droplet vitrification proved to be viable for the long-term storage of mangaba tree samples. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1679-9275 1807-8621 |
| Relation: | http://186.233.154.254/ojs/index.php/ActaSciAgron/article/view/19573; https://doaj.org/toc/1679-9275; https://doaj.org/toc/1807-8621; https://doaj.org/article/d2725738848645bb92e3123f0ede06d9 |
| DOI: | 10.4025/actasciagron.v37i3.19573 |
| الاتاحة: | https://doi.org/10.4025/actasciagron.v37i3.19573 https://doaj.org/article/d2725738848645bb92e3123f0ede06d9 |
| رقم الانضمام: | edsbas.4FB9E5FA |
| قاعدة البيانات: | BASE |
| تدمد: | 16799275 18078621 |
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| DOI: | 10.4025/actasciagron.v37i3.19573 |