Academic Journal
High-fat diet-induced obesity increases brain mitochondrial complex I and lipoxidation-derived protein damage
| العنوان: | High-fat diet-induced obesity increases brain mitochondrial complex I and lipoxidation-derived protein damage |
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| المؤلفون: | Berdún Hernández, Rebeca, Obis Monné, Èlia, Mota Martorell, Natàlia, Bassols, Anna, Valent, Daniel, Serrano Casasola, José Carlos Enrique, Martín Garí, Meritxell, Rodríguez Palmero, María, Moreno-Muñoz, José Antonio, Tibau, Joan, Quintanilla Aguado, Raquel, Pamplona Gras, Reinald, Portero Otín, Manuel, Jové Font, Mariona |
| بيانات النشر: | MDPI |
| سنة النشر: | 2024 |
| المجموعة: | Universitat de Lleida: Repositori Obert UdL |
| مصطلحات موضوعية: | Obesity, Lipoxidation, Mitochondrial complexes, n3 PUFA, Probiotics, Age-related neurodegenerative diseases |
| الوصف: | Obesity is a risk factor for highly prevalent age-related neurodegenerative diseases, the pathogenesis of whichinvolves mitochondrial dysfunction and protein oxidative damage. Lipoxidation, driven by high levels of peroxidizable unsaturated fatty acids and low antioxidant protection of the brain, stands out as a significant risk factor. To gain information on the relationship between obesity and brain molecular damage, in a porcine model of obesity we evaluated (1) the level of mitochondrial respiratory chain complexes, as the main source of free radical generation, by Western blot; (2) the fatty acid profile by gas chromatography; and (3) the oxidative modification of proteins by mass spectrometry. The results demonstrate a selectively higher amount of the lipoxidation-derived biomarker malondialdehyde-lysine (MDAL) (34% increase) in the frontal cortex, and positive correlations between MDAL and LDL levels and body weight. No changes were observed in brain fatty acid profile by the high-fat diet, and the increased lipid peroxidative modification was associated with increased levels of mitochondrial complex I (NDUFS3 and NDUFA9 subunits) and complex II (flavoprotein). Interestingly, introducing n3 fatty acids and a probiotic in the high-fat diet prevented the observed changes, suggesting that dietary components can modulate protein oxidative modification at the cerebral level and opening new possibilities in neurodegenerative diseases' prevention. ; This research was supported by Laboratorios Ordesa S.L., Center for Technological and Industrial Development (CDTI) through the SMARTFOODS PROJECT (IDI 20141216), Fondo Europeo de Desarrollo Regional (FEDER; Acronym: INCOMES, project number IPT-20111008), and ISCIII [PI20-000155] to M.P.O. This work was also supported by the Generalitat of Catalonia (Agency for Management of University and Research Grants [2021SGR00990]) to R.P. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-3921 |
| Relation: | https://doi.org/10.3390/antiox13020161; Antioxidants, 2024, vol. 13, núm. 2, p. 161; https://repositori.udl.cat/handle/10459.1/465635 |
| DOI: | 10.3390/antiox13020161 |
| الاتاحة: | https://doi.org/10.3390/antiox13020161 https://repositori.udl.cat/handle/10459.1/465635 |
| Rights: | cc-by (c) authors, 2024 ; info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.4EE259BC |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20763921 |
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| DOI: | 10.3390/antiox13020161 |