التفاصيل البيبلوغرافية
| العنوان: |
Effects of cytochalasin B and D upon insulin release and pancreatic islet cell metabolism. |
| المؤلفون: |
Jijakli, Hassan, Zhang, Hai-Xia, Dura, Eszter, Ramirez, Remedios, Sener, Abdullah, Malaisse, Willy |
| المصدر: |
International Journal of Molecular Medicine, 9 (2 |
| سنة النشر: |
2002 |
| المجموعة: |
DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
| مصطلحات موضوعية: |
Sciences bio-médicales et agricoles, 3-O-Methylglucose -- metabolism, Amino Acids -- metabolism, Animals, Carbon Dioxide -- metabolism, Cytochalasin B -- pharmacology, Cytochalasin D -- pharmacology, Forskolin -- pharmacology, Glucose -- metabolism, Glutamine -- pharmacology, Insulin -- secretion, Islets of Langerhans -- drug effects, Islets of Langerhans -- metabolism, Islets of Langerhans -- secretion, Keto Acids -- pharmacology, Leucine -- pharmacology, Radioisotopes, Rats, Water -- metabolism |
| الوصف: |
Cytochalasin B is known to enhance insulin release evoked by nutrient and non-nutrient secretagogues, including D-glucose, despite inhibiting D-glucose uptake and metabolism in pancreatic islets. In the present study, cytochalasin D, which failed to affect D-glucose uptake and metabolism by isolated islets, also augmented glucose-stimulated insulin release, but unexpectedly to a lesser extent than cytochalasin B. Such was not the case, however, in islets stimulated by non-glucidic nutrients such as 2-ketoisocaproate or the association of L-leucine and L-glutamine. This situation coincided with the fact that cytochalasin B inhibited more severely D-glucose metabolism in non-B, as distinct from B, islet cells and, in the former case, caused a relatively greater inhibition of hexose catabolism at 2.8 mM than at 16.7 mM D-glucose. Nevertheless, even in the presence of forskolin, cytochalasin B was more efficient than cytochalasin D in augmenting glucose-stimulated insulin secretion. Thus, although these data document that non-B islet cells are more sensitive than purified islet B cells to cytochalasin B, at least in terms of inhibition of D-glucose catabolism, such a difference and its possible consequence upon the release of glucagon and other non-insulinic hormones by non-B islet cells do not appear sufficient to account for the greater enhancing action of cytochalasin B, as distinct from cytochalasin D, upon glucose-stimulated insulin output. Likewise, the latter difference does not appear attributable to a greater efficiency of cytochalasin B, as compared to cytochalasin D, upon the mechanical events involved in nutrient-stimulated exocytosis of insulin granules. Hence, the present findings suggest a so-far-unidentified interference of cytochalasin B with the B-cell glucose-sensing device. ; Journal Article ; Research Support, Non-U.S. Gov't ; info:eu-repo/semantics/published |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
No full-text files |
| اللغة: |
English |
| Relation: |
uri/info:pmid/11786928; uri/info:scp/0036484065; http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/68636 |
| الاتاحة: |
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/68636 |
| رقم الانضمام: |
edsbas.4E95ADDD |
| قاعدة البيانات: |
BASE |