Academic Journal

In vitro characterization of [(125)I]HY-3-24, a selective ligand for the dopamine D3 receptor

التفاصيل البيبلوغرافية
العنوان: In vitro characterization of [(125)I]HY-3-24, a selective ligand for the dopamine D3 receptor
المؤلفون: Lee, Ji Youn, Kim, Ho Young, Martorano, Paul, Riad, Aladdin, Taylor, Michelle, Luedtke, Robert R., Mach, Robert H.
المصدر: Frontiers in Neuroscience
بيانات النشر: Frontiers Media S.A.
سنة النشر: 2024
المجموعة: UNTHSC Scholarly Repository (University. of North Texas Health Science Center)
مصطلحات موضوعية: [125i]hy-3-24, autoradiography, dopamine D3 receptor ligand, in vitro binding assay, islands of Calleja, nonhuman primates
الوصف: INTRODUCTION: Dopamine D3 receptor (D3R) ligands have been studied for the possible treatment of neurological and neuropsychiatric disorders. However, selective D3R radioligands for in vitro binding studies have been challenging to identify due to the high structural similarity between the D2R and D3R. In a prior study, we reported a new conformationally-flexible benzamide scaffold having a high affinity for D3R and excellent selectivity vs. D2R. In the current study, we characterized the in vitro binding properties of a new radioiodinated ligand, [(125)I]HY-3-24. METHODS: In vitro binding studies were conducted in cell lines expressing D3 receptors, rat striatal homogenates, and rat and non-human primate (NHP) brain tissues to measure regional brain distribution of this radioligand. RESULTS: HY-3-24 showed high potency at D3R (K(i) = 0.67 +/- 0.11 nM, IC(50) = 1.5 +/- 0.58 nM) compared to other D2-like dopamine receptor subtypes (D2R K(i) = 86.7 +/- 11.9 nM and D4R K(i) > 1,000). The K(d) (0.34 +/- 0.22 nM) and B(max) (38.91 +/- 2.39 fmol/mg) values of [(125)I]HY-3-24 were determined. In vitro binding studies in rat striatal homogenates using selective D2R and D3R antagonists confirmed the D3R selectivity of [(125)I]HY-3-24. Autoradiography results demonstrated that [(125)I]HY-3-24 specifically binds to D3Rs in the nucleus accumbens, islands of Calleja, and caudate putamen in rat and NHP brain sections. CONCLUSION: These results suggest that [(125)I]HY-3-24 appears to be a novel radioligand that exhibits high affinity binding at D3R, with low binding to other D2-like dopamine receptors. It is anticipated that [(125)I]HY-3-24 can be used as the specific D3R radioligand. ; The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by the National Institute on Drug Abuse (grant number DA029840).
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: unknown
تدمد: 1662-4548
Relation: https://doi.org/10.3389/fnins.2024.1380009; Lee, J. Y., Kim, H. Y., Martorano, P., Riad, A., Taylor, M., Luedtke, R. R., & Mach, R. H. (2024). In vitro characterization of [125I]HY-3-24, a selective ligand for the dopamine D3 receptor. Frontiers in neuroscience, 18, 1380009. https://doi.org/10.3389/fnins.2024.1380009; https://hdl.handle.net/20.500.12503/32815; 18
الاتاحة: https://hdl.handle.net/20.500.12503/32815
Rights: Attribution 4.0 International (CC BY 4.0 DEED) ; http://creativecommons.org/licenses/by/4.0/ ; © 2024 Lee, Kim, Martorano, Riad, Taylor, Luedtke and Mach.
رقم الانضمام: edsbas.4E869EAE
قاعدة البيانات: BASE