Academic Journal
Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging
| العنوان: | Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging |
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| المؤلفون: | Gilleron, Jérôme, Meziat, Cindy, Sulen, André, Ivanov, Stoyan, Jager, Jennifer, Estève, David, Muller, Catherine, Tanti, Jean-Francois, Cormont, Mireille |
| المساهمون: | Université Côte D'Azur, Inserm, Centre Méditerranéen de Médecine Moleculaire( C3M), Team « Cellular and Molecular Pathophysiology of Obesity and Diabetes », Department of Medicine Karolinska Institute, Karolinska University Hospital Stockholm, Université Côte d'Azur, Inserm, Centre Méditerranéen de Médecine Moléculaire (C3M), Team "Haematometabolism in Diseases", Institut de pharmacologie et de biologie structurale (IPBS), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), ANR-18-CE14-0035,Rab4LiverMetabolism,Déterminer le rôle de l'endocytose dépendante de Rab4b dans la régulation du métabolisme hépatique du glucose(2018) |
| المصدر: | ISSN: 1940-087X. |
| بيانات النشر: | HAL CCSD JoVE |
| سنة النشر: | 2020 |
| المجموعة: | Université Toulouse III - Paul Sabatier: HAL-UPS |
| مصطلحات موضوعية: | Adipose tissue, clearing, light microscopy, 3-D whole tissue, obesity, morphology, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism |
| الوصف: | International audience ; Obesity is a major worldwide public health issue that increases the risk to develop cardiovascular diseases, type-2 diabetes, and liver diseases. Obesity is characterized by an increase in adipose tissue (AT) mass due to adipocyte hyperplasia and/or hypertrophia, leading to profound remodeling of its three-dimensional structure. Indeed, the maximal capacity of AT to expand during obesity is pivotal to the development of obesity-associated pathologies. This AT expansion is an important homeostatic mechanism to enable adaptation to an excess of energy intake and to avoid deleterious lipid spillover to other metabolic organs, such as muscle and liver. Therefore, understanding the structural remodeling that leads to the failure of AT expansion is a fundamental question with high clinical applicability. In this article, we describe a simple and fast clearing method that is routinely used in our laboratory to explore the morphology of mouse and human white adipose tissue by fluorescent imaging. This optimized AT clearing method is easily performed in any standard laboratory equipped with a chemical hood, a temperature-controlled orbital shaker and a fluorescent microscope. Moreover, the chemical compounds used are readily available. Importantly, this method allows one to resolve the 3D AT structure by staining various markers to specifically visualize the adipocytes, the neuronal and vascular networks, and the innate and adaptive immune cells distribution. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-03033057; https://hal.science/hal-03033057; https://hal.science/hal-03033057/document; https://hal.science/hal-03033057/file/Gilleron%20et%20al.,%20JoVE2020%20submitted%20revised%20version.pdf |
| DOI: | 10.3791/61640 |
| الاتاحة: | https://hal.science/hal-03033057 https://hal.science/hal-03033057/document https://hal.science/hal-03033057/file/Gilleron%20et%20al.,%20JoVE2020%20submitted%20revised%20version.pdf https://doi.org/10.3791/61640 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.4E61F854 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3791/61640 |
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