Academic Journal
Antigen specific mammary inflammation depends on the production of IL-17A and IFN-γ by bovine CD4+ T lymphocytes
| العنوان: | Antigen specific mammary inflammation depends on the production of IL-17A and IFN-γ by bovine CD4+ T lymphocytes |
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| المؤلفون: | Rainard, Pascal, Cunha, Patricia, Ledresseur, Marion, Staub, Christophe, Touze, Jean-Luc, Kempf, Florent, Gilbert, Florence B., Foucras, Gilles |
| المساهمون: | Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements Nouzilly (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation Saumur (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Interactions hôtes-agents pathogènes Toulouse (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), Université de Toulouse (UT), Region Centre (Mamimmunotypage project) 2012 00073634, National Institute for Agricultural Research (INRA) |
| المصدر: | ISSN: 1932-6203. |
| بيانات النشر: | HAL CCSD Public Library of Science |
| سنة النشر: | 2015 |
| المجموعة: | Université François-Rabelais de Tours: HAL |
| مصطلحات موضوعية: | immunologic reactions, T-lymphocyte, mammary gland, antigen, inflammation mammaire, réponse immunitaire, bovin, lymphocyte t, glande mammaire, antigène, échographie, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.IMM]Life Sciences [q-bio]/Immunology |
| الوصف: | Remerciements au LEGTA de Vendôme, Loir-et-Cher ; Intramammary infusion of the antigen used to sensitize cows by the systemic route induces a local inflammation associated with neutrophil recruitment. We hypothesize that this form of delayed type hypersensitivity, which may occur naturally during infections or could be induced intentionally by vaccination, can impact the outcome of mammary gland infections. We immunized cows with ovalbumin to identify immunological correlates of antigen-specific mammary inflammation. Intraluminal injection of ovalbumin induced a mastitis characterized by a prompt tissue reaction (increase in teat wall thickness) and an intense influx of leukocytes into milk of 10 responder cows out of 14 immunized animals. The magnitude of the local inflammatory reaction, assessed through milk leukocytosis, correlated with antibody titers, skin thickness test, and production of IL-17A and IFN-γ in a whole-blood antigen stimulation assay (WBA). The production of these two cytokines significantly correlated with the magnitude of the milk leukocytosis following the ovalbumin intramammary challenge. The IL-17A and IFN-γ production in the WBA was dependent on the presence of CD4+ cells in blood samples. In vitro stimulation of peripheral blood lymphocytes with ovalbumin followed by stimulation with PMA/ionomycin allowed the identification by flow cytometry of CD4+ T cells producing either IL-17A, IFN-γ, or both cytokines. The results indicate that the antigen-specific WBA, and specifically IL-17A and IFN-γ production by circulating CD4+ cells, can be used as a predictor of mammary hypersensitivity to protein antigens. This prompts further studies aiming at determining how Th17 and/or Th1 lymphocytes modulate the immune response of the mammary gland to infection. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/26375594; PRODINRA: 325139; PUBMED: 26375594; WOS: 000361610200051 |
| DOI: | 10.1371/journal.pone.0137755 |
| الاتاحة: | https://hal.science/hal-01595331 https://hal.science/hal-01595331v1/document https://hal.science/hal-01595331v1/file/2015_PRainard_PlosOne_%7BE61CDCB1-D456-47A0-8826-1A91A5945170%7D.pdf https://doi.org/10.1371/journal.pone.0137755 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.4E000BA6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0137755 |
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