Academic Journal
Correlation between degree of rupture of outer mitochondrial membrane and changes of kinetics of regulation of respiration by ADP in permeabilized heart and liver cells.
| العنوان: | Correlation between degree of rupture of outer mitochondrial membrane and changes of kinetics of regulation of respiration by ADP in permeabilized heart and liver cells. |
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| المؤلفون: | Saks, Valdur, A, Belikova, Y., Vasilyeva, E., Kuznetsov, Andrei, V., Fontaine, Eric, M., Keriel, Christiane, M., Leverve, Xavier, M |
| المساهمون: | Bioénergétique fondamentale et appliquée, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cardiol Ctr, Bioenerget Grp |
| المصدر: | ISSN: 0006-291X. |
| بيانات النشر: | CCSD Elsevier |
| سنة النشر: | 1995 |
| المجموعة: | Université Grenoble Alpes: HAL |
| مصطلحات موضوعية: | MESH: Adenosine Diphosphate, MESH: Animals, MESH: Mitochondrial Swelling, MESH: Myocardium, MESH: Oxygen Consumption, MESH: Permeability, MESH: Rats, MESH: Cells, Cultured, MESH: Homeostasis, MESH: Intracellular Membranes, MESH: Kinetics, MESH: Liver, MESH: Microscopy, Electron, MESH: Mitochondria, Heart, Liver, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology |
| الوصف: | International audience ; Kinetics of regulation of respiration by ADP in skinned cardiac fibers and permeabilized isolated hepatocytes was compared before and after mitochondrial swelling due to hypoosmotic treatment in 30-50 mOsM solutions. In both systems the apparent Km for ADP was high before mitochondrial swelling and equal to 297 +/- 35 microM and 275 +/- 35 microM, correspondingly. Hypoosmotic treatment resulted in the rupture of outer mitochondrial membrane and in the loss of exogenous cytochrome c, and both in skinned cardiac fibers and permeabilized hepatocytes the value of apparent Km for ADP was decreased in correlation with the extent of the rupture of the outer mitochondrial membrane. It is concluded that both in heart and liver cells in vivo, the ADP diffusion in the cells is retarded due to very low permeability of the outer mitochondrial membrane for ADP. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/7702621; PUBMED: 7702621 |
| DOI: | 10.1006/bbrc.1995.1422 |
| الاتاحة: | https://inserm.hal.science/inserm-00390253 https://doi.org/10.1006/bbrc.1995.1422 |
| رقم الانضمام: | edsbas.4DB559AD |
| قاعدة البيانات: | BASE |
| DOI: | 10.1006/bbrc.1995.1422 |
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