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Aging induces hepatic oxidative stress and nuclear proteomic remodeling in liver from wistar rats

التفاصيل البيبلوغرافية
العنوان: Aging induces hepatic oxidative stress and nuclear proteomic remodeling in liver from wistar rats
المؤلفون: Bárcena, Brenda, Salamanca, Aurora, Pintado, Cristina, Mazuecos, Lorena, Villar, Margarita, Moltó, Eduardo, Bonzón-Kulichenko, Elena, Vázquez, Jesús, Andrés, Antonio, Gallardo, Nilda
المساهمون: Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Universidad de Castilla La Mancha
بيانات النشر: Multidisciplinary Digital Publishing Institute
سنة النشر: 2021
المجموعة: Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council)
مصطلحات موضوعية: Oxidative stress, RNA splicing, Aging, Liver proteome, Aging-associated chronic diseases
الوصف: This article belongs to the Special Issue Oxidative Stress in Aging and Associated Chronic Diseases. ; Aging is a continuous, universal, and irreversible process that determines progressive loss of adaptability. The liver is a critical organ that supports digestion, metabolism, immunity, detoxification, vitamin storage, and hormone signaling. Nevertheless, the relationship between aging and the development of liver diseases remains elusive. In fact, although prolonged fasting in adult rodents and humans delays the onset of the disease and increases longevity, whether prolonged fasting could exert adverse effects in old organisms remains incompletely understood. In this work, we aimed to characterize the oxidative stress and nuclear proteome in the liver of 3-month- and 24-month-old male Wistar rats upon 36 h of fasting and its adaptation in response to 30 min of refeeding. To this end, we analyzed the hepatic lipid peroxidation levels (TBARS) and the expression levels of genes associated with fat metabolism and oxidative stress during aging. In addition, to gain a better insight into the molecular and cellular processes that characterize the liver of old rats, the hepatic nuclear proteome was also evaluated by isobaric tag quantitation (iTRAQ) mass spectrometry-based proteomics. In old rats, aging combined with prolonged fasting had great impact on lipid peroxidation in the liver that was associated with a marked downregulation of antioxidant genes (Sod2, Fmo3, and Cyp2C11) compared to young rats. Besides, our proteomic study revealed that RNA splicing is the hepatic nuclear biological process markedly affected by aging and this modification persists upon refeeding. Our results suggest that aged-induced changes in the nuclear proteome could affect processes associated with the adaptative response to refeeding after prolonged fasting, such as those involved in the defense against oxidative stress. ; This work was supported by Research Grant RTI2018-098643-B-I00 from Ministerio de Ciencia, Innovación y ...
نوع الوثيقة: article in journal/newspaper
اللغة: English
تدمد: 2076-3921
Relation: #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098643-B-I00/ES/ANALISIS TEMPORAL DEL DESARROLLO DE RESISTENCIA HIPOTALAMICA A LEPTINA: CONSECUENCIAS SOBRE LA ADIPOSIDAD Y LA INFLAMACION HIPOTALAMICA Y PERIFERICA/; Publisher's version; https://doi.org/10.3390/antiox10101535; Sí; Antioxidants 10(10): 1535 (2021); http://hdl.handle.net/10261/259340; http://dx.doi.org/10.13039/501100007480; http://dx.doi.org/10.13039/501100011033; http://dx.doi.org/10.13039/501100000780
DOI: 10.3390/antiox10101535
DOI: 10.13039/501100007480
DOI: 10.13039/501100011033
DOI: 10.13039/501100000780
الاتاحة: http://hdl.handle.net/10261/259340
https://doi.org/10.3390/antiox10101535
https://doi.org/10.13039/501100007480
https://doi.org/10.13039/501100011033
https://doi.org/10.13039/501100000780
Rights: open
رقم الانضمام: edsbas.4D72A672
قاعدة البيانات: BASE
الوصف
تدمد:20763921
DOI:10.3390/antiox10101535