التفاصيل البيبلوغرافية
| العنوان: |
Sperm DNA Fragmentation and Sperm-Borne miRNAs: Molecular Biomarkers of Embryo Development? |
| المؤلفون: |
Anna Chiara Conflitti, Gaia Cicolani, Alessandra Buonacquisto, Francesco Pallotti, Fabiana Faja, Serena Bianchini, Giovanna Blaconà, Sabina Maria Bruno, Antonella Linari, Marco Lucarelli, Diletta Montanino, Ludovico Muzii, Andrea Lenzi, Francesco Lombardo, Donatella Paoli |
| المصدر: |
International Journal of Molecular Sciences; Volume 24; Issue 2; Pages: 1007 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2023 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
miR-34c-5p, miR-449b-5p, Sperm DNA Fragmentation, sperm-borne miRNA, Intracytoplasmic Sperm Injection, embryo development |
| جغرافية الموضوع: |
agris |
| الوصف: |
The evaluation of morpho-functional sperm characteristics alone is not enough to explain infertility or to predict the outcome of Assisted Reproductive Technologies (ART): more sensitive diagnostic tools are needed in clinical practice. The aim of the present study was to analyze Sperm DNA Fragmentation (SDF) and sperm-borne miR-34c-5p and miR-449b-5p levels in men of couples undergoing ART, in order to investigate any correlations with fertilization rate, embryo quality and development. Male partners (n = 106) were recruited. Semen analysis, SDF evaluation and molecular profiling analysis of miR-34c-5p and miR-449b-5p (in 38 subjects) were performed. Sperm DNA Fragmentation evaluation- a positive correlation between SDF post sperm selection and the percentage of low-quality embryos and a negative correlation with viable embryo were found. SDF > 2.9% increased the risk of obtaining a non-viable embryo by almost 4-fold. Sperm miRNAs profile—we found an association with both miRNAs and sperm concentration, while miR-449b-5p is positively associated with SDF. Moreover, the two miRNAs are positively correlated. Higher levels of miR-34c-5p compared to miR-449b-5p increases by 14-fold the probability of obtaining viable embryos. This study shows that SDF, sperm miR-34c-5p, and miR-449b-5p have a promising role as biomarkers of semen quality and ART outcome. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Molecular Biology; https://dx.doi.org/10.3390/ijms24021007 |
| DOI: |
10.3390/ijms24021007 |
| الاتاحة: |
https://doi.org/10.3390/ijms24021007 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.4CF4E068 |
| قاعدة البيانات: |
BASE |