Academic Journal
The Lack of WIP Binding to Actin Results in Impaired B Cell Migration and Altered Humoral Immune Responses
| العنوان: | The Lack of WIP Binding to Actin Results in Impaired B Cell Migration and Altered Humoral Immune Responses |
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| المؤلفون: | Keppler, SJ, Burbage, M, Gasparrini, F, Hartjes, L, Aggarwal, S, Massaad, MJ, Geha, RS, Bruckbauer, A, Batista, FD |
| المصدر: | 629 ; 619 |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2018 |
| المجموعة: | Imperial College London: Spiral |
| الوصف: | Wiskott-Aldrich syndrome protein (WASp) is a main cytoskeletal regulator in B cells. WASp-interacting protein (WIP) binds to and stabilizes WASp but also interacts with actin. Using mice with a mutated actin binding domain of WIP (WIPΔABD), we here investigated the role of WIP binding to actin during B cell activation. We found an altered differentiation of WIPΔABD B cells and diminished antibody affinity maturation after immunization. Mechanistically, WIPΔABD B cells showed impaired B cell receptor (BCR)-induced PI3K signaling and actin reorganization, likely caused by diminished CD81 expression and altered CD19 dynamics on the B cell surface. WIPΔABD B cells displayed reduced in vivo motility, concomitantly with impaired chemotaxis and defective F-actin polarization, HS1 phosphorylation, and polarization of HS1 to F-actin-rich structures after CXCL12 stimulation in vitro. We thus concluded that WIP binding to actin, independent of its binding to WASp, is critical for actin cytoskeleton plasticity in B cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | Cell Reports; http://hdl.handle.net/10044/1/62258; https://dx.doi.org/10.1016/j.celrep.2018.06.051 |
| DOI: | 10.1016/j.celrep.2018.06.051 |
| الاتاحة: | http://hdl.handle.net/10044/1/62258 https://doi.org/10.1016/j.celrep.2018.06.051 |
| Rights: | © 2018 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| رقم الانضمام: | edsbas.4CF1D344 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.celrep.2018.06.051 |
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