Academic Journal
14-3-3 protein interacts with and affects the structure of RGS domain of regulator of G protein signaling 3 (RGS3)
| العنوان: | 14-3-3 protein interacts with and affects the structure of RGS domain of regulator of G protein signaling 3 (RGS3) |
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| المؤلفون: | Řežábková, L., Bouřa, E., Herman, P., Večeř, J., Bouřová, L. (Lenka), Šulc, M. (Miroslav), Svoboda, P. (Petr), Obšilová, V. (Veronika), Obšil, T. |
| سنة النشر: | 2010 |
| المجموعة: | The Czech Academy of Sciences: Publication Activity (ASEP) / Akademie věd ČR - Publikační činnost |
| مصطلحات موضوعية: | 14-3-3 protein, time-resolved fluorescence, RGS3 |
| الوصف: | We have investigated whether the 14-3-3 protein binding affects the structure of RGS3 using the time-resolved tryptophan fluorescence spectroscopy and X-ray protein crystallography. Our results revealed that the 14-3-3 protein binding induces structural changes in both the N-terminal part and the C-terminal RGS domain of phosphorylated RGS3 molecule. The data obtained from the resolution of the crystal structure of the RGS domain suggest that the 14-3-3 protein-induced conformational change affects the region within the G(alpha)-interacting portion of the RGS domain. This can explain the inhibitory effect of the 14-3-3 protein on GAP activity of RGS3 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1047-8477 1095-8657 |
| Relation: | urn:pissn: 1047-8477; urn:eissn: 1095-8657; http://hdl.handle.net/11104/0185864 |
| DOI: | 10.1016/j.jsb.2010.03.009 |
| الاتاحة: | https://doi.org/10.1016/j.jsb.2010.03.009 http://hdl.handle.net/11104/0185864 |
| رقم الانضمام: | edsbas.4CC67F93 |
| قاعدة البيانات: | BASE |
| تدمد: | 10478477 10958657 |
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| DOI: | 10.1016/j.jsb.2010.03.009 |