Academic Journal
Intra-Articular Injections of the Adipose-Derived Mesenchymal Stem Cells Suppress Progression of a Mouse Traumatic Knee Osteoarthritis Model
| العنوان: | Intra-Articular Injections of the Adipose-Derived Mesenchymal Stem Cells Suppress Progression of a Mouse Traumatic Knee Osteoarthritis Model |
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| المؤلفون: | Wakayama, Takanori, Saita, Yoshitomo, Nagao, Masashi, Uchino, Sayuri, Yoshihara, Sei-ichi, Tsuji, Kunikazu, Koga, Hideyuki, Kobayashi, Yohei, Nishio, Hirofumi, Momoi, Yasumasa, Ikeda, Hiroshi, Kaneko, Kazuo, Ishijima, Muneaki |
| المساهمون: | BioMimetics Sympathies |
| المصدر: | CARTILAGE ; volume 13, issue 4, page 148-156 ; ISSN 1947-6035 1947-6043 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2022 |
| الوصف: | Objective This study aimed to determine whether the intra-articular injection of human adipose-derived mesenchymal stem cells (ADSCs) protects against the progression of murine post-traumatic osteoarthritis. Design ADSCs were isolated from human abdomen or buttock adipose tissues. In in vitro study, ADSCs conditioned medium was added to human chondrocytes pre-treated with interleukin-1β (IL-1β), and resultant gene expression of target inflammatory genes was measured by real-time quantitative polymerase chain reaction. A mouse model of knee osteoarthritis was generated by unilaterally transecting the medial meniscus in the right hind limb of 20 female C57BL/6 mice. Mice were randomly assigned to 2 treatment groups that received 6 µl intra-articular injections of either phosphate-buffered saline (control) or 2 × 10 4 cells/μl of ADSCs 14, 28, and 42 days post-surgery. Mice were euthanized 84 days post-surgery and histological and micro-computed tomography evaluation of knee joints were analyzed. Hind limb weight-bearing distribution was measured pre-surgery and 28 and 84 days post-surgery. Results Conditioned medium from cultured human adipose-derived mesenchymal stem cells suppressed the expression of target inflammatory genes in chondrocytes pre-treated with IL-1β, suggesting anti-inflammatory properties ( P < 0.01). Histological analyses indicated that the progression of destabilization of medial meniscus-induced knee osteoarthritis was suppressed by the administration of ADSCs compared with control group at medial femorotibial joint in vivo. This protective effect was related to a reduction in articular cartilage loss. Conclusion The intra-articular injection of ADSCs suppressed articular cartilage loss in a mouse model of knee osteoarthritis, possible through anti-inflammatory mechanisms. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/19476035221132262 |
| الاتاحة: | https://doi.org/10.1177/19476035221132262 http://journals.sagepub.com/doi/pdf/10.1177/19476035221132262 http://journals.sagepub.com/doi/full-xml/10.1177/19476035221132262 |
| Rights: | https://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.4CB150AA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/19476035221132262 |
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