التفاصيل البيبلوغرافية
| العنوان: |
EGF stimulation results in increased viral transcription in the HPV16(+) NIKS-SG3 cell line. |
| المؤلفون: |
Adrian J. Luna (10024591), Rosa T. Sterk (10024594), Anastacia M. Griego-Fisher (10024597), Joon-Yong Chung (573772), Kiersten L. Berggren (10024600), Virginie Bondu (5050838), Pamela Barraza-Flores (8700915), Andrew T. Cowan (10024603), Gregory N. Gan (10024606), Emrullah Yilmaz (10024609), Hanbyoul Cho (573770), Jae-Hoon Kim (442860), Stephen M. Hewitt (190750), Julie E. Bauman (5063948), Michelle A. Ozbun (10024612) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Medicine, Cell Biology, Genetics, Molecular Biology, Biotechnology, Developmental Biology, Cancer, Mental Health, Hematology, Infectious Diseases, Virology, papillomavirus oncogene expression, HPV oncogene expression, hr-HPV oncogene expression, neoplastic phenotype, hr-HPV oncogene transcription, transcription factor activity, epithelial contact inhibition, HPV-induced tumors Intracellular pa., stratifying epithelial tissues, EGFR, Mechanistic studies show, MEK, neoplastic tissue biopsy materials, tissue differentiation cues, ERK, epithelial organotypic tissue models, AP, replicative cycles |
| الوصف: |
(A-C) Cells were incubated with (+) or without (Ø) 10 ng/ml EGF for the indicated times. Total RNA was subject to RT-qPCR for quantification of HPV early transcripts E6 (A), E7 (B) or E1^E4/E5 (C) (primer placement shown in S1 Fig ). HPV mRNA levels were normalized to ribosomal protein 18s mRNA levels and are shown relative to the HPV mRNA levels in the 24h untreated cells for each experiment. The scatter plot shows the mean and range of the data from three independent experiments, wherein color-coded symbols show the data from independent experiments. The data were analyzed using 1-way ANOVA with Dunnett’s T3 multiple comparison test post hoc (not significant [n.s.]). (D-F) RNA ISH specific for hr-HPV E6/E7 RNAs on cells cultured with 10ng/ml EGF; (G-I) dose-effect of EGF on cells grown for 24h with or without added EGF (G-I). Bars equal 100 μm. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/EGF_stimulation_results_in_increased_viral_transcription_in_the_HPV16_NIKS-SG3_cell_line_/13631743 |
| DOI: |
10.1371/journal.ppat.1009216.g002 |
| الاتاحة: |
https://doi.org/10.1371/journal.ppat.1009216.g002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.4C97E448 |
| قاعدة البيانات: |
BASE |