Academic Journal
Regulation of later neurogenic stages of adult‐derived neural stem/progenitor cells by L‐type Ca 2+ channels
| العنوان: | Regulation of later neurogenic stages of adult‐derived neural stem/progenitor cells by L‐type Ca 2+ channels |
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| المؤلفون: | Teh, Daniel B. L., Ishizuka, Toru, Yawo, Hiromu |
| المصدر: | Development, Growth & Differentiation ; volume 56, issue 8, page 583-594 ; ISSN 0012-1592 1440-169X |
| بيانات النشر: | Wiley |
| سنة النشر: | 2014 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | In the adult hippocampus, new neurons are continuously generated and incorporated into the local circuitry in a manner dependent on the network activity. Depolarization evoked by neurotransmitters has been assumed to activate L ‐type C a 2+ channels ( LTCC ) which regulate the intracellular C a 2+ ‐dependent signaling cascades. The process of neurogenesis contains several stages such as proliferation, fate determination, selective death/survival and maturation. Here, we investigated which stage of neurogenesis is under the regulation of LTCC using a clonal line of neural stem/progenitor cells, PZ 5, which was derived from adult rat hippocampus. Although undifferentiated PZ 5 cells were type 1‐like cells expressing both nestin and glial fibrillary acidic protein, they generated neuronal, astrocytic and oligodendrocytic populations in differentiation medium containing retinoic acid. Proliferation of undifferentiated PZ 5 cells was dependent on neither the LTCC antagonist, nimodipine (Nimo) nor the LTCC agonists, Bay K 8644 (BayK) or FPL 64176 ( FPL ), whereas the fraction of neuronal population that expressed both β III ‐tubulin and MAP 2 was reduced by Nimo but increased by BayK or FPL . At an earlier period of differentiation (e.g. day 4), the fraction of PZ 5 cells expressing HuC/D, pan‐neuronal marker, was not affected either by the LTCC activation or inhibition. At a later period of differentiation (e.g. day 9), the fraction of dying neurons was decreased by LTCC activation and increased by LTCC inhibition. It is suggested that the LTCC activation facilitates the survival and maturation of immature neurons, and that its inhibition facilitates the neuronal death. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/dgd.12158 |
| الاتاحة: | http://dx.doi.org/10.1111/dgd.12158 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fdgd.12158 https://onlinelibrary.wiley.com/doi/pdf/10.1111/dgd.12158 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.4C917F47 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/dgd.12158 |
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