Academic Journal
A guide to time-resolved structural analysis of light-activated proteins
| العنوان: | A guide to time-resolved structural analysis of light-activated proteins |
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| المؤلفون: | Poddar, Harshwardhan, Heyes, Derren, Schirò, Giorgio, Weik , Martin, Leys, David, Scrutton, Nigel |
| المصدر: | Poddar , H , Heyes , D , Schirò , G , Weik , M , Leys , D & Scrutton , N 2022 , ' A guide to time-resolved structural analysis of light-activated proteins ' , The FEBS Journal , vol. 289 , no. 3 , pp. 576-595 . https://doi.org/10.1111/febs.15880 |
| سنة النشر: | 2022 |
| المجموعة: | The University of Manchester: Research Explorer - Publications |
| مصطلحات موضوعية: | light-activated proteins, photoenzymes, photoreceptors, protein dynamics, SAXS, time-resolved, TR-SFX, WAXS, ResearchInstitutes_Networks_Beacons/manchester_institute_of_biotechnology, Manchester Institute of Biotechnology |
| الوصف: | Dynamical changes in protein structures are essential for protein function and occur over femtoseconds to seconds timescales. X-ray free electron lasers have facilitated investigations of structural dynamics in proteins with unprecedented temporal and spatial resolution. Light-activated proteins are attractive targets for time-resolved structural studies, as the reaction chemistry and associated protein structural changes can be triggered by short laser pulses. Proteins with different light-absorbing centres have evolved to detect light and harness photon energy to bring about downstream chemical and biological output responses. Following light absorption, rapid chemical/small-scale structural changes are typically localised around the chromophore. These localised changes are followed by larger structural changes propagated throughout the photoreceptor/photocatalyst that enables the desired chemical and/or biological output response. Time-resolved serial femtosecond crystallography (SFX) and solution scattering techniques enable direct visualisation of early chemical change in light-activated proteins on timescales previously inaccessible, whereas scattering gives access to slower timescales associated with more global structural change. Here, we review how advances in time-resolved SFX and solution scattering techniques have uncovered mechanisms of photochemistry and its coupling to output responses. We also provide a prospective on how these time-resolved structural approaches might impact on other photoreceptors/photoenzymes that have not yet been studied by these methods. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/febs.15880 |
| الاتاحة: | https://research.manchester.ac.uk/en/publications/17a77c8e-f327-41be-90e8-50cb15e43a20 https://doi.org/10.1111/febs.15880 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.4C4F18E4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/febs.15880 |
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