Academic Journal
1,25-dihydroxyvitamin D 3 ameliorates high glucose-mediated proliferation, migration, and MCP-1 secretion of vascular smooth muscle cells by inhibiting MAPK phosphorylation
| العنوان: | 1,25-dihydroxyvitamin D 3 ameliorates high glucose-mediated proliferation, migration, and MCP-1 secretion of vascular smooth muscle cells by inhibiting MAPK phosphorylation |
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| المؤلفون: | Kaizu, Xu, Ying, Wu, Mei-fang, Wu, Li-ming, Lin |
| المصدر: | Journal of International Medical Research ; volume 50, issue 9 ; ISSN 0300-0605 1473-2300 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2022 |
| الوصف: | Objectives To explore the impacts of 1,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) on the proliferation, migration, and monocyte chemoattractant protein-1 (MCP-1) secretion of vascular smooth muscle cells (VSMCs) in a high glucose environment and its possible mechanism. Methods We extracted VSMCs from the thoracic aorta of a male Sprague–Dawley rats before culturing them in a 25-mM glucose-containing medium in the presence or absence of 1,25(OH) 2 D 3 (10 −9 –10 −7 M). Cell proliferation was determined by bromodeoxyuridine incorporation assays. Subsequently, cell migratory capacity was examined by performing a transwell assay. An enzyme-linked immunosorbent assay was conducted to assess MCP-1 levels. Protein levels of matrix metalloproteinase-9 (MMP-9), mitogen-activated protein kinases (MAPKs), cyclin D1, and phosphorylated MAPKs were determined by immunoblotting. Results 1,25(OH) 2 D 3 significantly suppressed the proliferation, migration, and MCP-1 secretion of VSMCs mediated by high glucose in a dose-dependent manner, diminished the enhanced protein expression of MMP-9 and cyclin D1, and attenuated MAPK phosphorylation. The p38 inhibitor SB203580 and ERK1/2 inhibitor PD98059 suppressed high glucose-mediated upregulation of MMP-9 and cyclin D1 protein expression and MCP-1 secretion, respectively. Conclusions 1,25(OH) 2 D 3 ameliorates high glucose-mediated proliferation, migration, and MCP-1 secretion of VSMCs by inhibiting MAPK phosphorylation, implying a potential therapeutic approach using 1,25(OH) 2 D 3 for diabetic macrovascular complications. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/03000605221121973 |
| الاتاحة: | https://doi.org/10.1177/03000605221121973 https://journals.sagepub.com/doi/pdf/10.1177/03000605221121973 https://journals.sagepub.com/doi/full-xml/10.1177/03000605221121973 |
| Rights: | https://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.4BB39A32 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/03000605221121973 |
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