Academic Journal
IGF-I binding in primary culture of muscle cells of rainbow trout: changes during in vitro development
| العنوان: | IGF-I binding in primary culture of muscle cells of rainbow trout: changes during in vitro development |
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| المؤلفون: | Castillo, Juan, Le Bail, Pierre-Yves, Paboeuf, Gilles, Navarro, Isabel, Weil, Claudine, Fauconneau, Benoit, Gutiérrez, Joaquim |
| المساهمون: | Universitat de Barcelona (UB), Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA) |
| المصدر: | ISSN: 0363-6119. |
| بيانات النشر: | CCSD American Physiological Society |
| سنة النشر: | 2002 |
| المجموعة: | Institut National de la Recherche Agronomique: ProdINRA |
| مصطلحات موضوعية: | CULTURE DE CELLULE, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | International audience ; To characterize and study the variations of IGF-I binding during the development of trout muscle cells, in vitro experiments were conducted using myocyte cultures, and IGF-I binding assays were performed in three stages of cell development: mononuclear cells (day 1), small myotubes (day 4), and large myotubes (day 10). Binding experiments were done by incubating cells with IGF-I for 12 h at 4°C. Specific IGF-I binding increased with the concentration of labeled IGF-I and reached a plateau at 32 pM. The displacement of cold human and trout IGF-I showed a very similar curve (EC50 = 1.19 ± 0.05 and 0.95 ± 0.05 nM, respectively). IGF binding proteins did not interfere significantly because displacement of labeled IGF-I by either cold trout recombinant IGF-I or Des (1–3) IGF-I resulted in similar curves. Insulin did not displace labeled IGF-I even at very high concentrations (>1 μM), which indicates the specificity of IGF-I binding. The amount of receptor (R0) increased from 253 ± 51 fmol/mg DNA on day 1 to 766 ± 107 fmol/mg DNA onday 10. However, the affinity (Kd) of IGF-I receptors did not change significantly during this development (from 1.29 ± 0.19 to 0.79 ± 0.13 nM). On the basis of our results, we conclude that rainbow trout muscle cells in culture express specific IGF-I receptors, which increase their number with development from mononuclear cells to large myotubes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-177490-2 0-00-177490-5 |
| Relation: | PRODINRA: 64201; WOS: 000177490500013 |
| DOI: | 10.1152/ajpregu.00121.2002 |
| الاتاحة: | https://hal.inrae.fr/hal-02677784 https://hal.inrae.fr/hal-02677784v1/document https://hal.inrae.fr/hal-02677784v1/file/64201_20100908103353149_1.pdf https://doi.org/10.1152/ajpregu.00121.2002 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.4BAA14B3 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780001774902 0001774905 |
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| DOI: | 10.1152/ajpregu.00121.2002 |