التفاصيل البيبلوغرافية
| العنوان: |
Comparative Cytotoxicity of Five Current Dentin Bonding Agents: Role of Cell Cycle Deregulation ; 五種牙本質黏著劑毒性之比較: 細胞週期異常之角色 |
| المؤلفون: |
葉宏偉, 張美姬, 林俊彬, 曾琬瑜, 張曉華, 王東美, 陳羿貞, 林秋君, 楊婷婷, 林立德, 鄭景暉, YEH, HUNG-WEI, CHANG, MEI-CHI, LIN, CHUN- PIN, TSENG, WAN-YU, CHANG, HSIAO-HUA, WANG, TONG-MEI, CHEN, YI-JANE, LIN, CHIU-CHUN, YANG, TING-TING, LIN, LI-DEH, JENG, JIIANG-HUEI |
| المساهمون: |
牙醫學系所 |
| سنة النشر: |
2009 |
| المجموعة: |
National Taiwan University Institutional Repository (NTUR) |
| الوصف: |
To compare the cytotoxicity of three nano-dentin bonding agents (nano- DBAs) and two non-nano-DBAs using Chinese hamster ovary (CHO-K1) cells. We found that nano fillers were not the major contributing factor in DBA cytotoxicity, as analyzed by colony forming assay and 3-(4,5-dimethyl- thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Exposure of CHO-K1 cells to all three tested total-etching DBAs led to G(0)/G(1) cell cycle arrest, whereas exposure to higher concentrations of two tested nano -DBAs induced G(2)/ M arrest. All five DBAs further induced apoptosis at the highest concentration, as analyzed by propidium iodide staining flow cytometry. The toxicity of all DBAs (1:4000v/v or higher) is related to increased reactive oxygen speciesROS) production, as analyzed by single cell DCF fluorescence flow cytometry. These results indicate that clinical application of DBAs may be potentially toxic to dental pulp tissues. Cytotoxicity of DBAs is associated with ROS production, cell cycle deregulation and apoptosis. Presence of methacrylate monomers such as PENTA and UDMA is possibly the major cytotoxic factor for DBAs. Further studies on other toxicological endpoints of nano-DBAs are necessary to highlight their safe use. staining flow cytometry. The toxicity of all DBAs (1:4000v/v or higher) is related to increased reactive oxygen species |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
ACTA BIOMATERIALIA v.5 n.9 pp.3404-3410; http://ntur.lib.ntu.edu.tw/handle/246246/185860 |
| الاتاحة: |
http://ntur.lib.ntu.edu.tw/handle/246246/185860 |
| رقم الانضمام: |
edsbas.4B8F4A62 |
| قاعدة البيانات: |
BASE |