Academic Journal
Encapsulation of peptides in biodegradable microspheres prolongs their MHC class-I presentation by dendritic cells and macrophages in vitro
| العنوان: | Encapsulation of peptides in biodegradable microspheres prolongs their MHC class-I presentation by dendritic cells and macrophages in vitro |
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| المؤلفون: | Audran, R., Peter, K., Dannull, J., Men, Y., Scandella, E., Groettrup, M., Gander, B., Corradin, G. |
| المصدر: | Vaccine, vol. 21, no. 11-12, pp. 1250-5 |
| سنة النشر: | 2003 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: | Adjuvants, Immunologic Animals *Antigen Presentation Antigens/*administration & dosage/immunology Antigens, Protozoan/administration & dosage/immunology Antigens, Viral/administration & dosage/immunology Cells, Cultured/immunology Dendritic Cells/*immunology Drug Compounding Epitopes, T-Lymphocyte/*administration & dosage/immunology Humans Influenza A virus/immunology Interferon Type II/secretion Kinetics Lactic Acid Macrophages/*immunology Mice Microspheres Peptide Fragments/administration & dosage/immunology Plasmodium berghei/immunology Plasmodium falciparum/immunology Polyglycolic Acid Polymers Protozoan Proteins/administration & dosage/immunology T-Lymphocytes, Cytotoxic/immunology/secretion Vaccines, Subunit/*administration & dosage/immunology Viral Matrix Proteins/immunology |
| الوصف: | Biodegradable microspheres (MS) consisting of poly(D,L-lactide-co-glycolide) (PLGA) represent a promising alternative to conventional adjuvants. The adjustable pulsatile release of encapsulated material from such MS offers the potential to mimic the priming and boosting injections of conventional immunization regimens. In this paper, we demonstrate that MS can serve as antigen reservoirs in antigen presenting cells (APC), so that antigen is presented for extended periods of time (up to 9 days). In particular, we could show by measurement of IFN-gamma production that encapsulated peptides were presented to cytotoxic T lymphocytes (CTL) by mouse and human macrophages as well as by human dendritic cells in vitro for a longer time period as compared to soluble peptides. The extended antigen presentation may thus improve the CTL response in vivo. These results may be of paramount importance in cancer vaccination therapy since MS may serve as antigen reservoirs to extend the presentation time by APC used to boost the patient's immune response to tumor antigens. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/12559806; info:eu-repo/semantics/altIdentifier/pissn/0264-410X; https://serval.unil.ch/notice/serval:BIB_EF0B0D971D04 |
| DOI: | 10.1016/S0264-410X(02)00521-2 |
| الاتاحة: | https://serval.unil.ch/notice/serval:BIB_EF0B0D971D04 https://doi.org/10.1016/S0264-410X(02)00521-2 |
| رقم الانضمام: | edsbas.4B2022BD |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/S0264-410X(02)00521-2 |
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