Academic Journal
Field evaluation of a locally produced rapid diagnostic test for early detection of cholera in Bangladesh.
| العنوان: | Field evaluation of a locally produced rapid diagnostic test for early detection of cholera in Bangladesh. |
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| المؤلفون: | Md Taufiqul Islam, Ashraful Islam Khan, Md Abu Sayeed, Jakia Amin, Kamrul Islam, Nur Alam, Nishat Sultana, Noor Jahan, Md Mahbubur Rashid, Zahid Hasan Khan, Mazharul Islam Zion, Mokibul Hassan Afrad, Shah Alam Siddique, Farhana Khanam, Yasmin Ara Begum, Muhammad Shariful Islam, Firdausi Qadri |
| المصدر: | PLoS Neglected Tropical Diseases, Vol 13, Iss 1, p e0007124 (2019) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2019 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270 |
| الوصف: | BACKGROUND:Cholera remains a substantial health burden in Asia and Africa particularly in resource poor settings. The standard procedures to identify the etiological organism V. cholerae are isolation from microbiological culture from stool as well as Polymerase Chain Reaction (PCR). Both the processes are highly lab oriented, labor extensive, time consuming, and expensive. In an effort to control for outbreaks and epidemics; an effective, convenient, quick and relatively less expensive detection method is imperative, without compromising the sensitivity and specificity that exists at present. The objective of this component of the study was to evaluate the effectiveness of a locally produced rapid diagnostic test (RDT) for cholera diagnosis. METHODS:In Bangladesh, nationwide cholera surveillance is ongoing in 22 hospitals covering all 8 divisions of the country since June, 2016. In the surveillance, stool samples have been collected from patients presenting to hospitals with acute watery diarrhea. Crystal VCTM (Span diagnostics, India) and Cholkit (locally produced RDT) have been used to detect V. cholerae from stool samples. Samples have also been sent to the main laboratory at icddr,b where the culture based isolation is routinely performed. All the tests were carried out for both direct and enriched stool samples. RDT sensitivity and specificity were calculated using stool culture as the gold standard. RESULTS:A total of 7720 samples were tested. Among these, 5865 samples were solely tested with Crystal VC and 1355 samples with Cholkit whereas 381 samples were tested with both the RDTs. In comparison with culture, direct testing with Crystal VC showed a sensitivity of 72% (95% CI: 50.6% to 87.9%) and specificity of 86.8% (95% CI: 82.8% to 90.1%). After enrichment the sensitivity and specificity was 68% (95% CI: 46.5% to 85.1%) and 97.5% (95% CI: 95.3% to 98.8%) respectively. The direct Cholkit test showed sensitivity of 76% (95% CI: 54.9% to 90.6%) and specificity of 90.2% (95% CI: 86.6% to 93.1%). ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1935-2727 1935-2735 |
| Relation: | http://europepmc.org/articles/PMC6372204?pdf=render; https://doaj.org/toc/1935-2727; https://doaj.org/toc/1935-2735; https://doaj.org/article/81d75d12ea0a420381078e7de2387789 |
| DOI: | 10.1371/journal.pntd.0007124 |
| الاتاحة: | https://doi.org/10.1371/journal.pntd.0007124 https://doaj.org/article/81d75d12ea0a420381078e7de2387789 |
| رقم الانضمام: | edsbas.4AFA256C |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19352727 19352735 |
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| DOI: | 10.1371/journal.pntd.0007124 |