Academic Journal
Compression and crevasses in vitreous sections under different cutting conditions.
| العنوان: | Compression and crevasses in vitreous sections under different cutting conditions. |
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| المؤلفون: | Han, H.M., Zuber, B., Dubochet, J. |
| المصدر: | Journal of microscopy, vol. 230, no. Pt 2, pp. 167-171 |
| سنة النشر: | 2008 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: | Artifacts, Cryoelectron Microscopy/methods, Cryoultramicrotomy/instrumentation, Cryoultramicrotomy/methods, Enterococcus faecalis/growth & development, Enterococcus faecalis/ultrastructure, Frozen Sections |
| الوصف: | Compression and crevasses are common cutting artefacts in cryo-ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent methods, was quantitatively related to feed and compression. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/18445144; info:eu-repo/semantics/altIdentifier/eissn/1365-2818; https://serval.unil.ch/notice/serval:BIB_3E8894A1BA88 |
| DOI: | 10.1111/j.1365-2818.2008.01972.x |
| الاتاحة: | https://serval.unil.ch/notice/serval:BIB_3E8894A1BA88 https://doi.org/10.1111/j.1365-2818.2008.01972.x |
| رقم الانضمام: | edsbas.4AD7D3A1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1365-2818.2008.01972.x |
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