التفاصيل البيبلوغرافية
| العنوان: |
The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties |
| المؤلفون: |
Io Antonopoulou, Adiphol Dilokpimol, Laura Iancu, Miia Mäkelä, Simona Varriale, Gabriella Cerullo, Silvia Hüttner, Stefan Uthoff, Peter Jütten, Alexander Piechot, Alexander Steinbüchel, Lisbeth Olsson, Vincenza Faraco, Kristiina Hildén, Ronald De Vries, Ulrika Rova, Paul Christakopoulos |
| المصدر: |
Catalysts; Volume 8; Issue 6; Pages: 242 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2018 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
transesterification, feruloyl esterase, antioxidants, phylogenetic classification, prenyl ferulate, prenyl caffeate, glyceryl ferulate, butyl ferulate, l -arabinose ferulate, sugar esters |
| الوصف: |
Twenty-eight fungal feruloyl esterases (FAEs) were evaluated for their synthetic abilities in a ternary system of n-hexane: t-butanol: 100 mM MOPS-NaOH pH 6.0 forming detergentless microemulsions. Five main derivatives were synthesized, namely prenyl ferulate, prenyl caffeate, butyl ferulate, glyceryl ferulate, and l-arabinose ferulate, offering, in general, higher yields when more hydrophilic alcohol substitutions were used. Acetyl xylan esterase-related FAEs belonging to phylogenetic subfamilies (SF) 5 and 6 showed increased synthetic yields among tested enzymes. In particular, it was shown that FAEs belonging to SF6 generally transesterified aliphatic alcohols more efficiently while SF5 members preferred bulkier l-arabinose. Predicted surface properties and structural characteristics were correlated with the synthetic potential of selected tannase-related, acetyl-xylan-related, and lipase-related FAEs (SF1-2, -6, -7 members) based on homology modeling and small molecular docking simulations. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Biocatalysis; https://dx.doi.org/10.3390/catal8060242 |
| DOI: |
10.3390/catal8060242 |
| الاتاحة: |
https://doi.org/10.3390/catal8060242 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.4AC47368 |
| قاعدة البيانات: |
BASE |