Academic Journal
NFI-A directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls β-globin and G-CSF receptor expression
| العنوان: | NFI-A directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls β-globin and G-CSF receptor expression |
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| المؤلفون: | STARNES LM, SORRENTINO A, PELOSI E, BALLARINO, MONICA, MORSILLI O, BIFFONI M, SANTORO S, FELLI N, CASTELLI G, DE MARCHIS, MARIA LAURA, MASTROBERARDINO G, GABBIANELLI M, FATICA, Alessandro, BOZZONI, Irene, NERVI, Clara, PESCHLE C. |
| المساهمون: | Starnes, Lm, Sorrentino, A, Pelosi, E, Ballarino, Monica, Morsilli, O, Biffoni, M, Santoro, S, Felli, N, Castelli, G, DE MARCHIS, MARIA LAURA, Mastroberardino, G, Gabbianelli, M, Fatica, Alessandro, Bozzoni, Irene, Nervi, Clara, Peschle, C. |
| بيانات النشر: | AMER SOC HEMATOLOGY |
| سنة النشر: | 2009 |
| المجموعة: | Sapienza Università di Roma: CINECA IRIS |
| مصطلحات موضوعية: | antigen, beta-globin, biological, biosynthesi, cd34, cell differentiation, cell lineage, cytology, erythrocyte, erythropoietin, fetal blood, gene expression regulation, genetic, granulocyte colony-stimulating factor, granulocyte, hematopoietic stem cell, human, metabolism, model, nfi transcription factor, promoter region, receptors |
| الوصف: | It is generally conceded that selective combinations of transcription factors determine hematopoietic lineage commitment and differentiation. Here we show that in normal human hematopoiesis the transcription factor nuclear factor I-A (NFI-A) exhibits a marked lineage-specific expression pattern: it is upmodulated in the erythroid (E) lineage while fully suppressed in the granulopoietic (G) series. In unilineage E culture of hematopoietic progenitor cells (HPCs), NFI-A overexpression or knockdown accelerates or blocks erythropoiesis, respectively: notably, NFI-A overexpression restores E differentiation in the presence of low or minimal erythropoietin stimulus. Conversely, NFI-A ectopic expression in unilineage G culture induces a sharp inhibition of granulopoiesis. Finally, in bilineage E + G culture, NFI-A overexpression or suppression drives HPCs into the E or G differentiation pathways, respectively. These NFI-A actions are mediated, at least in part, by a dual and opposite transcriptional action: direct binding and activation or repression of the promoters of the beta-globin and G-CSF receptor gene, respectively. Altogether, these results indicate that, in early hematopoiesis, the NFI-A expression level acts as a novel factor channeling HPCs into either the E or G lineage. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/19542302; info:eu-repo/semantics/altIdentifier/wos/WOS:000269380600010; volume:114; issue:9; firstpage:1753; lastpage:1763; numberofpages:11; journal:BLOOD; http://hdl.handle.net/11573/230626; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-70349242211; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000269380600010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a; http://www.scopus.com/inward/record.url?eid=2-s2.0-70349242211&partnerID=65&md5=cd8147ceaa2caf835b3165e4f3b94acd |
| DOI: | 10.1182/blood-2008-12-196196 |
| الاتاحة: | http://hdl.handle.net/11573/230626 https://doi.org/10.1182/blood-2008-12-196196 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000269380600010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-70349242211&partnerID=65&md5=cd8147ceaa2caf835b3165e4f3b94acd |
| رقم الانضمام: | edsbas.4ABA44D1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1182/blood-2008-12-196196 |
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