Academic Journal
Characteristics of splenic CD8+ T cell exhaustion in patients with hepatitis C
| العنوان: | Characteristics of splenic CD8+ T cell exhaustion in patients with hepatitis C |
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| المؤلفون: | K. Sumida, S. Shimoda, S. Iwasaka, S. Hisamoto, H. Kawanaka, T. Akahoshi, T. Ikegami, K. Shirabe, N. Shimono, Y. Maehara, M. E. Gershwin, K. Akashi, C. Selmi |
| المساهمون: | K. Sumida, S. Shimoda, S. Iwasaka, S. Hisamoto, H. Kawanaka, T. Akahoshi, T. Ikegami, K. Shirabe, N. Shimono, Y. Maehara, C. Selmi, M.E. Gershwin, K. Akashi |
| سنة النشر: | 2013 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | Hepatitis C, Liver cirrhosi, PD-1, Portal hypertension, Splenectomy, Tim-3, Aged, Antigen-Presenting Cell, Biological Marker, CD8-Positive T-Lymphocyte, Female, Chronic, Human, Male, Membrane Protein, Middle Aged, Programmed Cell Death 1 Receptor, Spleen, Thrombocytopenia, Immunology, Immunology and Allergy, Settore MED/04 - Patologia Generale |
| الوصف: | Summary: There is increasing interest in the role of T cell exhaustion and it is well known that the natural history of chronic hepatitis C virus infection (HCV) is modulated by CD8+ T cell immunobiology. There are many pathways that alter the presence of exhaustive T cells and, in particular, they are functionally impaired by inhibitory receptors, such as programmed death-1 (PD-1) and T cell immunoglobulin and mucin domain-containing protein 3 (Tim-3). We obtained spleen, liver and peripheral blood (before and after splenectomy) lymphoid cells from 25 patients with HCV-related cirrhosis undergoing liver transplantation for end-stage disease or splenectomy for portal hypertension. In all samples we performed an extensive phenotypic study of exhaustion markers [PD-1, Tim-3, interferon (IFN)-γ) and their ligands (PD-L1, PD-L2, galectin-9] in CD8+ T cell subpopulations (both total and HCV-specific) and in antigen-presenting cells (APC; monocytes and dendritic cells). In the spleen, total and HCV-specific CD8+ T cells demonstrated enhanced markers of exhaustion, predominantly in the effector memory subpopulation. Similarly, splenic APC over-expressed inhibitory receptor ligands when compared to peripheral blood. Finally, when peripheral blood CD8+ T cells were compared before and after splenectomy, markers of exhaustion were reduced in splenic CD8+ T cells and APC. Our data in HCV-related cirrhosis suggest that CD8+ T cells in the spleen manifest a significantly higher exhaustion compared to peripheral blood and may thus contribute to the failure to control HCV. Counteracting this process may contribute to inducing an effective immune response to HCV. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/23773130; info:eu-repo/semantics/altIdentifier/wos/WOS:000324045900018; volume:174; issue:1; firstpage:172; lastpage:178; numberofpages:7; journal:CLINICAL AND EXPERIMENTAL IMMUNOLOGY; http://hdl.handle.net/2434/252401; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84883624028 |
| DOI: | 10.1111/cei.12158 |
| الاتاحة: | http://hdl.handle.net/2434/252401 https://doi.org/10.1111/cei.12158 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.4A9DDBC6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/cei.12158 |
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