Academic Journal
Pre-Transplant Expression of CCR-2 in Kidney Transplant Recipients Is Associated With the Development of Delayed Graft Function
| العنوان: | Pre-Transplant Expression of CCR-2 in Kidney Transplant Recipients Is Associated With the Development of Delayed Graft Function |
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| المؤلفون: | Pontrelli, Paola, Simone, Simona, Rascio, Federica, Pesce, Francesco, Conserva, Francesca, Infante, Barbara, Castellano, Giuseppe, Sallustio, Fabio, Fiorentino, Marco, Zaza, Gianluigi, Gallone, Anna, Battaglia, Michele, Ditonno, Pasquale, Stallone, Giovanni, Gesualdo, Loreto, Grandaliano, Giuseppe |
| المصدر: | Frontiers in Immunology ; volume 13 ; ISSN 1664-3224 |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2022 |
| المجموعة: | Frontiers (Publisher - via CrossRef) |
| الوصف: | Background Delayed graft function (DGF) leads to a reduced graft survival. Donors’ features have been always considered as key pathogenic factors in this setting. The aim of our study was to evaluate the recipients’ characteristics in the development of DGF. Methods We enrolled 932 kidney graft recipients from 466 donors; 226 recipients experienced DGF. In 290 donors, both recipients presented with early graft function (EGF, group A), in 50 both recipients experienced DGF (group B), and in 126 one recipient presented with DGF and the other with EGF (group C). In group C, we selected 7 couples of DGF/EGF recipients and we evaluated the transcriptomic profile by microarray on circulating mononuclear cells harvested before transplantation. Results were validated by qPCR in an independent group of 25 EGF/DGF couples. Findings In the whole study group, DGF was associated with clinical characteristics related to both donors and recipient. In group C, DGF was significantly associated with body mass index, hemodialysis, and number of mismatches. In the same group, we identified 411 genes differently expressed before transplantation between recipients discordant for the transplant outcome. Those genes were involved in immune dysfunction and inflammation. In particular, we observed a significant increase in DGF patients in the expression of C–C chemokine receptor type 2 (CCR2), the monocyte chemoattractant protein-1 (MCP-1) receptor. CCR-2 upregulation was confirmed in an independent cohort of patients. Conclusions Our results suggest that recipients’ clinical/immunological features, potentially modulated by dialysis, are associated with the development of DGF independently of donors’ features. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.3389/fimmu.2022.804762 |
| DOI: | 10.3389/fimmu.2022.804762/full |
| الاتاحة: | http://dx.doi.org/10.3389/fimmu.2022.804762 https://www.frontiersin.org/articles/10.3389/fimmu.2022.804762/full |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.4A620755 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2022.804762 |
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