Academic Journal
Prognostic significance of AP-2α/γ targets as cancer therapeutics
| العنوان: | Prognostic significance of AP-2α/γ targets as cancer therapeutics |
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| المؤلفون: | Damian Kołat, Żaneta Kałuzińska, Andrzej K. Bednarek, Elżbieta Płuciennik |
| المصدر: | Scientific Reports, Vol 12, Iss 1, Pp 1-16 (2022) |
| بيانات النشر: | Nature Portfolio |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Identifying genes with prognostic importance could improve cancer treatment. An increasing number of reports suggest the existence of successful strategies based on seemingly “untargetable” transcription factors. In addition to embryogenesis, AP-2 transcription factors are known to play crucial roles in cancer development. Members of this family can be used as prognostic factors in oncological patients, and AP-2α/γ transcription factors were previously investigated in our pan-cancer comparative study using their target genes. The present study investigates tumors that were previously found similar with an emphasis on the possible role of AP-2 factors in specific cancer types. The RData workspace was loaded back to R environment and 3D trajectories were built via Monocle3. The genes that met the requirement of specificity were listed using top_markers(), separately for mutual and unique targets. Furthermore, the candidate genes had to meet the following requirements: correlation with AP-2 factor (through Correlation AnalyzeR) and validated prognostic importance (using GEPIA2 and subsequently KM-plotter or LOGpc). Eventually, the ROC analysis was applied to confirm their predictive value; co-dependence of expression was visualized via BoxPlotR. Some similar tumors were differentiated by AP-2α/γ targets with prognostic value. Requirements were met by only fifteen genes (EMX2, COL7A1, GRIA1, KRT1, KRT14, SLC12A5, SEZ6L, PTPRN, SCG5, DPP6, NTSR1, ARX, COL4A3, PPEF1 and TMEM59L); of these, the last four were excluded based on ROC curves. All the above genes were confronted with the literature, with an emphasis on the possible role played by AP-2 factors in specific cancers. Following ROC analysis, the genes were verified using immunohistochemistry data and progression-related signatures. Staining differences were observed, as well as co-dependence on the expression of e.g. CTNNB1, ERBB2, KRAS, SMAD4, EGFR or MKI67. In conclusion, prognostic value of targets suggested AP-2α/γ as candidates for novel cancer ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doi.org/10.1038/s41598-022-09494-1; https://doaj.org/toc/2045-2322; https://doaj.org/article/d89922a4e1bb45e48f108a5919d09c25 |
| DOI: | 10.1038/s41598-022-09494-1 |
| الاتاحة: | https://doi.org/10.1038/s41598-022-09494-1 https://doaj.org/article/d89922a4e1bb45e48f108a5919d09c25 |
| رقم الانضمام: | edsbas.4A10A15E |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-022-09494-1 |