Conference
Neuroprotective activity of GQD against SNP-induced toxicity are mediated by ROS/RNS scavenging and protective autophagy induction
| العنوان: | Neuroprotective activity of GQD against SNP-induced toxicity are mediated by ROS/RNS scavenging and protective autophagy induction |
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| المؤلفون: | Ristić, Biljana, Krunić, Matija, Bošnjak, Mihajlo, Mirčić, Aleksandar, Tovilović-Kovačević, Gordana, Zogović, Nevena, Paunović, Verica, Trajković, Vladimir, Harhaji-Trajković, Ljubica |
| المصدر: | 3rd Nordic Autophagy Society (NAS) Conference; 2019 May 22-24; Utrecht, Netherlands |
| بيانات النشر: | Nordic Autophagy Society |
| سنة النشر: | 2019 |
| المجموعة: | RADaR - Digital Repository of Archived Publications Institute for Biological Research "Sinisa Stankovic" / РАДаР - Репозиторијум Архивираних Дигиталних Радова Институт за биолошка истраживања "Синиша Станковић" |
| مصطلحات موضوعية: | neuroprotection, oxidative/nitrosative stress, autophagy, graphen quantum dots |
| الوصف: | We investigated the ability of nano-sized graphen layers graphen quantum dots (GQD) to protect human neuroblastoma SH-SY5Y cells from toxicity of NO donor sodium nitroprusside (SNP). GQD prevented SNP induced mitochondrial depolarization and caspase dependent apoptosis. GQD partly suppressed neurotoxicity of NO donor DEA-NONOate and reduced SNP induced NO release in cells and cell-free system, suggesting that neuroprotective effects of GQD were partly mediated by their NO-scavanging capacity. However, GQD significantly preserved SH-SY5Y cells from light exhausted SNP, which was unable to produce NO, implying the existence of protective mechanism independent of NO-scavenging. Unspecific antioxidant, as well as hydroxyl radical (.OH) scavengers DMSO, vitamin E and gluthatione mimicked neuroprotective activity of GQD, while GQD diminished concentration of reactive oxygen species (ROS), especially .OH, in cells and cell culture medium, suggesting important role of .OH scavenging in neuroprotective activity of GQD. However ability of GQD to protect SH-SY5Y cells from SNP was not exclusively mediated by their ability to scavenge NO and ROS from medium, since it persisted after washing of GQD preincubated cells. Interestingly, GQD were found to be present in autophagosome-like vacuoles. Both SNP and GQD, and especially their combination, increased intracellular acidity characteristic for presence of autophagosomes, concentration of proautophagic protein beclin-1, while deacreased level of specific substrate of autophagic proteolysis p62. Moreover, SNP and GQD, and above all their combination, increased concentration of autophagosome-associated protein LC3 II in the presence of inhibitor of autophagic proteolysis bafilomycin A1. Finally, autophagy inhibitors 3-methzladenine, wortmannin and NH4Cl prevented neuroprotective ability of GQD, implying that GQD stimulated prosurvival autophagy in SNP treated neurons. Therefore, by demonstrating ability of GQD to protect SH-SY5Y neurons from SNP induced apoptosis by scavenging ... |
| نوع الوثيقة: | conference object |
| اللغة: | English |
| Relation: | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41025/RS//; info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173053/RS//; https://nordicautophagy.org/; https://nordicautophagy.org/report-3rd-nordic-autophagy-society-conference-in-utrecht/; http://radar.ibiss.bg.ac.rs/handle/123456789/6718; https://radar.ibiss.bg.ac.rs/bitstream/id/16435/bitstream_16435.pdf; https://hdl.handle.net/21.15107/rcub_ibiss_6718 |
| الاتاحة: | https://nordicautophagy.org/ https://nordicautophagy.org/report-3rd-nordic-autophagy-society-conference-in-utrecht/ http://radar.ibiss.bg.ac.rs/handle/123456789/6718 https://radar.ibiss.bg.ac.rs/bitstream/id/16435/bitstream_16435.pdf https://hdl.handle.net/21.15107/rcub_ibiss_6718 |
| Rights: | openAccess ; ARR ; © 2019 by the Nordic Autophagy Society |
| رقم الانضمام: | edsbas.49E0D96B |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |