Academic Journal
Preconditioning with Foam-mediated External Suction on Flap Microvasculature and Perfusion in a Rodent Model
| العنوان: | Preconditioning with Foam-mediated External Suction on Flap Microvasculature and Perfusion in a Rodent Model |
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| المؤلفون: | Mohan, Anita T., Zhu, Lin, Michalak, Gregory J., Laungani, Alexis T., Vercnocke, Andrew J., Moran, Steven L., Saint-Cyr, Michel |
| المصدر: | Plastic and Reconstructive Surgery - Global Open ; volume 8, issue 8, page e2739 ; ISSN 2169-7574 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health) |
| سنة النشر: | 2020 |
| الوصف: | Background: Foam-mediated external suction (FMES) has previously shown to improve tissue microcirculation. We hypothesized that preconditioning fasciocutaneous perforator flaps with FMES would augment perfusion and demonstrate greater capillary recruitment. Methods: Gluteal perforator flaps were designed on sixteen 400 g rats. Continuous FMES at −125 mm Hg was applied on one side (intervention) to precondition tissue for 5 days, with the contralateral side as a paired control. In group A, we assessed changes following pretreatment, after surgery, and 7 days postprocedure, and in group B, we evaluated changes during preconditioning alone. In group A (N = 8), control and intervention flaps were assessed using laser-assisted indocyanine green fluorescence angiography. In group B, flap regions were assessed using 4-dimensional computed tomographic angiography. All flaps were analyzed for microvessel density using micro–computed tomography and histological assessment using hematoxylin and eosin and CD3 immunohistochemistry. Results: Thirty-two flaps were included in this study (N = 16 intervention and matched controls). Four-dimensional computed tomographic angiography demonstrated 17% greater tissue perfusion in preconditioned flaps (mean, 78.7 HU; SD, 8.8) versus controls (mean, 67.3 HU; SD, 15.7; P < 0.01). Laser-assisted indocyanine green fluorescence angiography showed a 30% higher mean absolute intensity in preconditioned flaps versus controls ( P < 0.01). Postsurgery mean absolute intensity in preconditioned flaps remained 21% higher than in controls ( P = 0.03). Preconditioned flaps demonstrated a 2-fold increase in mean vessel volume of 9.1 mm 3 (SD, 7) versus 4.5 mm 3 (SD, 3) in controls ( P = 0.04); there was a 33% higher mean area fraction of CD31 in preconditioned flaps, 3.9% (SD, 3) versus 2.9% (SD, 3) in controls ( P = 0.03). Conclusion: FMES preconditioning has the potential to augment vascularity of tissue for flap harvest; however, further experimental studies are required to optimize ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1097/gox.0000000000002739 |
| DOI: | 10.1097/GOX.0000000000002739 |
| الاتاحة: | http://dx.doi.org/10.1097/gox.0000000000002739 https://journals.lww.com/10.1097/GOX.0000000000002739 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.49C78596 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1097/gox.0000000000002739 |
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