التفاصيل البيبلوغرافية
| العنوان: |
Previously Unrecognized Nonreproducible Antibody–Probe Interactions |
| المؤلفون: |
Jiaojiao Pan (11972582), Lan Yang (457660), Wenya Wu (6559844), Jingzhi Li (41962), Hu Cheng (1349700), Yiting Li (2730601), Wenwen Xu (175846), Qinghong Xue (4796073), Youxin Zhou (5567549), Daxin Peng (305828), Jiwan Qiu (11972585), Hongwei Ma (290896) |
| سنة النشر: |
2022 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Medicine, Molecular Biology, Biotechnology, Evolutionary Biology, Ecology, Immunology, Marine Biology, Cancer, Mental Health, Plant Biology, Mathematical Sciences not elsewhere classified, exclusively accommodates noninteraction, ab – ag, encountered nonreproducible signals, state model ”), predictable yet certain, nonreproducible antibody, ultimately shows, substantial proportion, reproducible interaction, mab concentration, hook four, explain often, discoveries challenge, canonically described, based results |
| الوصف: |
Antibody–antigen (Ab–Ag) interactions are canonically described by a model that exclusively accommodates noninteraction (0) or reproducible interaction (RI) states, yet this model is inadequate to explain often-encountered nonreproducible signals. Here, by monitoring diverse experimental systems using a peptide–protein hybrid microarray, we observed that Ab–probe interactions comprise a substantial proportion of nonreproducible antibody-based results. This enabled our discovery and capacity to reliably identify nonreproducible Ab–probe interactions (NRIs), as well as our development of a powerful explanatory model (“0-NRI-RI-Hook four-state model”) that is mAb concentration-dependent, regardless of specificity, which ultimately shows that both nonspecific interactions and NRIs are not predictable yet certain to happen. Our discoveries challenge the centrality of Ab–Ag interaction specificity data in serology and immunology. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Previously_Unrecognized_Nonreproducible_Antibody_Probe_Interactions/18709862 |
| DOI: |
10.1021/acs.analchem.1c03264.s002 |
| الاتاحة: |
https://doi.org/10.1021/acs.analchem.1c03264.s002 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.49B52401 |
| قاعدة البيانات: |
BASE |