Academic Journal

A Sensitive and Rapid Method to Determine the Adhesion Capacity of Probiotics and Pathogenic Microorganisms to Human Gastrointestinal Mucins

التفاصيل البيبلوغرافية
العنوان: A Sensitive and Rapid Method to Determine the Adhesion Capacity of Probiotics and Pathogenic Microorganisms to Human Gastrointestinal Mucins
المؤلفون: Bélinda Ringot-Destrez, Zéa D’Alessandro, Jean-Marie Lacroix, Muriel Mercier-Bonin, Renaud Léonard, Catherine Robbe-Masselot
المصدر: Microorganisms; Volume 6; Issue 2; Pages: 49
بيانات النشر: Multidisciplinary Digital Publishing Institute
سنة النشر: 2018
المجموعة: MDPI Open Access Publishing
مصطلحات موضوعية: bacterial adhesion, mucin, O -glycosylation, sialic acids, probiotics, bacterial pathogens
جغرافية الموضوع: agris
الوصف: Mucus is the habitat for the microorganisms, bacteria and yeast that form the commensal flora. Mucins, the main macromolecules of mucus, and more specifically, the glycans that cover them, play essential roles in microbial gastrointestinal colonization. Probiotics and pathogens must also colonize mucus to have lasting positive or deleterious effects. The question of which mucin-harboured glycan motifs favour the adhesion of specific microorganisms remains very poorly studied. In the current study, a simple test based on the detection of fluorescent-labeled microorganisms raised against microgram amounts of mucins spotted on nitrocellulose was developed. The adhesion of various probiotic, commensal and pathogenic microorganisms was evaluated on a panel of human purified gastrointestinal mucins and compared with that of commercially available pig gastric mucins (PGM) and of mucins secreted by the colonic cancer cell line HT29-MTX. The latter two proved to be very poor indicators of adhesion capacity on intestinal mucins. Our results show that the nature of the sialylated cores of O-glycans, determined by MALDI MS-MS analysis, potentially enables sialic acid residues to modulate the adhesion of microorganisms either positively or negatively. Other identified factors affecting the adhesion propensity were O-glycan core types and the presence of blood group motifs. This test should help to select probiotics with enhanced adhesion capabilities as well as deciphering the role of specific mucin glycotopes on microbial adhesion.
نوع الوثيقة: text
وصف الملف: application/pdf
اللغة: English
Relation: Gut Microbiota; https://dx.doi.org/10.3390/microorganisms6020049
DOI: 10.3390/microorganisms6020049
الاتاحة: https://doi.org/10.3390/microorganisms6020049
Rights: https://creativecommons.org/licenses/by/4.0/
رقم الانضمام: edsbas.498911A6
قاعدة البيانات: BASE
الوصف
DOI:10.3390/microorganisms6020049