Academic Journal
Adipose TSHB in Humans and Serum TSH in Hypothyroid Rats Inform About Cellular Senescence
| العنوان: | Adipose TSHB in Humans and Serum TSH in Hypothyroid Rats Inform About Cellular Senescence |
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| المؤلفون: | Moreno-Navarrete, José María, Liñares-Pose, Laura, Sabater, Mónica, Rial-Pensado, Eva, Comas, Ferran, Jové Font, Mariona, Latorre, Jèssica, Ortega Delgado, Francisco José, Ricart, Wifredo, Portero Otín, Manuel, López, Miguel, Fernández Real, José Manuel |
| بيانات النشر: | Karger Publishers Open Access |
| سنة النشر: | 2018 |
| المجموعة: | Universitat de Lleida: Repositori Obert UdL |
| مصطلحات موضوعية: | Hipotiroïdisme, Hormones tiroides |
| الوصف: | Background/Aims: Thyroid hormones have been recently linked to senescence and longevity. Given the recent description of TSHB mRNA in human adipose tissue (AT), we aimed to investigate the relationship between local AT TSH and adipose tissue senescence. Methods: TSHB mRNA (measured by real-time PCR) and markers of adipose tissue senescence [BAX, DBC1, TP53, TNF (real-time PCR), telomere length (Telo TAGGG Telomere Length Assay) and lipidomics (liquid chromatography mass spectrometry)] were analysed in subcutaneous (SAT) and visceral (VAT) AT from euthyroid subjects. The chronic effects of TSH were also investigated in AT from hypothyroid rats and after recombinant human TSH (rhTSH) administration in human adipocytes. Results: Both VAT and SAT TSHB gene expression negatively correlated with markers of AT cellular senescence (BAX, DBC1, TP53, TNF gene expression and specific glucosylceramides) and positively associated with telomere length. Supporting these observations, both rhTSH administration in human adipocytes and increased TSH in hypothyroid rats resulted in decreased markers of cellular senescence (Bax and Tp53 mRNA) in both gonadal and subcutaneous white adipose tissue. Conclusion: These data point to a possible role of TSH in AT cellular senescence. ; This work was partially supported by research grant PI15/01934 and PI16/01173 from the Instituto de Salud Carlos III from Spain and was also supported by Fondo Europeo de Desarrollo Regional (FEDER); Xunta de Galicia (ML: 2015-CP079), Ministry of Economy and Competitiveness (ML: SAF2015-71026-R) and AtresMedia. CIBEROBN Fisiopatología de la Obesidad y Nutrición is an initiative from the Instituto de Salud Carlos III from Spain. We acknowledge the technical assistance of E. Loshuertos and O. Rovira (both from Endocrinology, IdIBGi, Spain). We want to particularly acknowledge the patients, the FATBANK platform promoted by the CIBEROBN and the IDIBGI Biobank (Biobanc IDIBGI, B.0000872), integrated in the Spanish National Biobanks Network, for their ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1015-8987 |
| Relation: | info:eu-repo/grantAgreement/MINECO//SAF2015-71026-R/ES/AMPK HIPOTALAMICA Y BALANCE ENERGETICO/; Reproducció del document publicat a: https://doi.org/10.1159/000495170; Cellular Physiology and Biochemistry, 2018, vol. 51, núm. 1, p. 142-153; http://hdl.handle.net/10459.1/65270; https://doi.org/10.1159/000495170 |
| DOI: | 10.1159/000495170 |
| الاتاحة: | http://hdl.handle.net/10459.1/65270 https://doi.org/10.1159/000495170 |
| Rights: | cc-by-nc-nd (c) José María Moreno-Navarrete et al., 2018 ; http://creativecommons.org/licenses/by-nc/3.0/es ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.4981201 |
| قاعدة البيانات: | BASE |
| تدمد: | 10158987 |
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| DOI: | 10.1159/000495170 |