التفاصيل البيبلوغرافية
| العنوان: |
Aminoacylation of hypomodified tRNA Glu in vivo |
| المؤلفون: |
Krüger, Malene Kappen, Sørensen, M.A. |
| المصدر: |
Krüger , M K & Sørensen , M A 1998 , ' Aminoacylation of hypomodified tRNA Glu in vivo ' , Journal of Molecular Biology , vol. 284 , no. 3 , pp. 609-620 . https://doi.org/10.1006/jmbi.1998.2197 |
| سنة النشر: |
1998 |
| مصطلحات موضوعية: |
archeo, envir |
| الوصف: |
Udgivelsesdato: 4 December 1998 ; The highly specific interaction of each aminoacyl-tRNA synthetase and its substrate tRNAs constitutes an intriguing problem in protein-RNA recognition. All tRNAs have the same overall three-dimensional structure in order to fit interchangeably into the translational apparatus. Thus, the recognition by aminoacyl-tRNA synthetase must be more or less limited to discrimination between bases at specific positions within the tRNA. The hypermodified nucleotide 5-methylaminomethyl-2-thiouridine (mnm 5 s 2 U) present at the wobble position of bacterial tRNAs specific for glutamic acid, lysine and possibly glutamine has been shown to be important in the recognition of these tRNAs by their synthetases in vitro . Here, we have determined the aminoacylation level in vivo of tRNA Glu , tRNA Lys , and tRNA 1 GIn in Escherichia coli strains containing undermodified derivatives of mnm 5 s 2 U34. Lack of the 5-methylaminomethyl group did not reduce charging levels for any of the three tRNAs. Lack of the s 2 U34 modification caused a 40% reduction in the charging level of tRNA Glu . Charging of tRNA Lys and tRNA 1 Gln were less affected. There was no compensating regulation of expression of glutamyl-tRNA synthetase because the relative synthesis rate was the same in the wild-type and mutant strains. These results indicate that the mnm 5 U34 modification is not an important recognition element in vivo for the glutamyl-tRNA synthetase. In contrast, lack of the s 2 U34 modification reduced the efficiency of charging by at least 40%. This is the minimal estimate because the turn-over rate of Glu-tRNA Glu was also reduced in the absence of the 2-thio group. Lack of either modification did not affect mischarging or mistranslation. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
https://curis.ku.dk/portal/da/publications/aminoacylation-of-hypomodified-trnaglu-in-vivo(c8fe0220-74c9-11db-bee9-02004c4f4f50).html |
| الاتاحة: |
https://curis.ku.dk/portal/da/publications/aminoacylation-of-hypomodified-trnaglu-in-vivo(c8fe0220-74c9-11db-bee9-02004c4f4f50).html |
| Rights: |
undefined |
| رقم الانضمام: |
edsbas.49249F9 |
| قاعدة البيانات: |
BASE |