Academic Journal
The Arabidopsis homolog of Scc4/MAU2 is essential for embryogenesis
| العنوان: | The Arabidopsis homolog of Scc4/MAU2 is essential for embryogenesis |
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| المؤلفون: | Minina, Elena A., Reza, Salim Hossaim, Gutiérrez-Beltrán, Emilio, Elander, Pernilla H., Bozhkov, Peter V., Moschou, Panagiotis N. |
| المساهمون: | The Erling-Persson Family Foundation, Swedish Research Council, Swedish Foundation for Strategic Research, Olle Engkvist Foundation, Knut and Alice Wallenberg Foundation, Carl Trygger Foundation |
| بيانات النشر: | Company of Biologists |
| سنة النشر: | 2017 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | MAU2, Arabidopsis, Auxin, Cohesin-loading complex, Scc4, Scc2, NIBPL, Embryogenesis |
| الوصف: | Factors regulating dynamics of chromatin structure have direct impact on expression of genetic information. Cohesin is a multi-subunit protein complex that is crucial for pairing sister chromatids during cell division, DNA repair and regulation of gene transcription and silencing. In non-plant species, cohesin is loaded on chromatin by the Scc2-Scc4 complex (also known as the NIBPL-MAU2 complex). Here, we identify the Arabidopsis homolog of Scc4, which we denote Arabidopsis thaliana (At)SCC4, and show that it forms a functional complex with AtSCC2, the homolog of Scc2. We demonstrate that AtSCC2 and AtSCC4 act in the same pathway, and that both proteins are indispensable for cell fate determination during early stages of embryo development. Mutant embryos lacking either of these proteins develop only up to the globular stage, and show the suspensor overproliferation phenotype preceded by ectopic auxin maxima distribution. We further establish a new assay to reveal the AtSCC4-dependent dynamics of cohesin loading on chromatin in vivo Our findings define the Scc2-Scc4 complex as an evolutionary conserved machinery controlling cohesin loading and chromatin structure maintenance, and provide new insight into the plant-specific role of this complex in controlling cell fate during embryogenesis. ; This work was supported by grants from Vetenskapsrådet (Swedish Research Council; to P.N.M. and P.V.B.), Pehrssons Fund (to P.V.B.), Stiftelsen för Strategisk Forskning (Swedish Foundation for Strategic Research (to P.V.B.), Stiftelsen Olle Engkvist Byggmästare (Olle Engkvist Foundation; to P.V.B.), the Knut och Alice Wallenbergs Stiftelse (Knut and Alice Wallenberg Foundation; to P.V.B.) and the Carl Tryggers Stiftelse för Vetenskaplig Forskning (Carl Tryggers Foundation; to E.A.M. and P.N.M.). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0021-9533 1477-9137 |
| Relation: | http://doi.org/10.1242/jcs.196865; Sí; Journal of Cell Science 130: 1051-1063 (2017); http://hdl.handle.net/10261/190031; http://dx.doi.org/10.13039/501100002805; http://dx.doi.org/10.13039/501100004063; http://dx.doi.org/10.13039/501100004200 |
| DOI: | 10.1242/jcs.19686 |
| DOI: | 10.13039/501100002805 |
| DOI: | 10.13039/501100004063 |
| DOI: | 10.13039/501100004200 |
| الاتاحة: | http://hdl.handle.net/10261/190031 https://doi.org/10.1242/jcs.19686 https://doi.org/10.13039/501100002805 https://doi.org/10.13039/501100004063 https://doi.org/10.13039/501100004200 |
| Rights: | none |
| رقم الانضمام: | edsbas.491E6E5F |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00219533 14779137 |
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| DOI: | 10.1242/jcs.19686 |