Academic Journal
Ether-Oxygen Containing Electrospun Microfibrous and Sub-Microfibrous Scaffolds Based on Poly(butylene 1,4-cyclohexanedicarboxylate) for Skeletal Muscle Tissue Engineering
| العنوان: | Ether-Oxygen Containing Electrospun Microfibrous and Sub-Microfibrous Scaffolds Based on Poly(butylene 1,4-cyclohexanedicarboxylate) for Skeletal Muscle Tissue Engineering |
|---|---|
| المؤلفون: | BLOISE, NORA, BERARDI, Emanuele, Gualandi, Chiara, Zaghi, Elisa, Duelen, Robin, Ceccarelli, Gabriele, Cortesi, Emanuela Elsa, Costamagna, Domiziana, Bruni, Giovanna, LOTTI, NADIA, Focarete, Maria Letizia, Visai, Livia, Sampaolesi, Maurilio |
| المساهمون: | orcid:0000-0002-6000-5036, orcid:0000-0002-0775-9605, #NODATA#, orcid:0000-0003-3899-0399, orcid:0000-0003-0904-1215, orcid:0000-0002-8576-694X, orcid:0000-0003-1958-2998, orcid:0000-0002-7976-2934, orcid:0000-0002-0458-7836, orcid:0000-0003-1181-3632, orcid:0000-0002-2422-3757 |
| بيانات النشر: | MDPI |
| سنة النشر: | 2021 |
| المجموعة: | Document Server@UHasselt (Universiteit Hasselt) |
| مصطلحات موضوعية: | biodegradable polyesters, electrospinning, microfibres and sub-microfibres, muscle tissue engineering, myogenesis, Animals, Cell Differentiation, Cell Line, Cell Proliferation, Cell Shape, Cyclohexanes, Implants, Experimental, Inflammation, Ki-67 Antigen, Male, Mice, Inbred C57BL, Muscle, Skeletal, Neovascularization, Physiologic, Oxygen, Polyenes, Polyethylene Glycols, Tissue Engineering, Tissue Scaffolds |
| الوصف: | We report the study of novel biodegradable electrospun scaffolds from poly(butylene 1,4-cyclohexandicarboxylate-co-triethylene cyclohexanedicarboxylate) (P(BCE-co-TECE)) as support for in vitro and in vivo muscle tissue regeneration. We demonstrate that chemical composition, i.e., the amount of TECE co-units (constituted of polyethylene glycol-like moieties), and fibre morphology, i.e., aligned microfibrous or sub-microfibrous scaffolds, are crucial in determining the material biocompatibility. Indeed, the presence of ether linkages influences surface wettability, mechanical properties, hydrolytic degradation rate, and density of cell anchoring points of the studied materials. On the other hand, electrospun scaffolds improve cell adhesion, proliferation, and differentiation by favouring cell alignment along fibre direction (fibre morphology), also allowing for better cell infiltration and oxygen and nutrient diffusion (fibre size). Overall, C2C12 myogenic cells highly differentiated into mature myotubes when cultured on microfibres realised with the copolymer richest in TECE co-units (micro-P73 mat). Lastly, when transplanted in the tibialis anterior muscles of healthy, injured, or dystrophic mice, micro-P73 mat appeared highly vascularised, colonised by murine cells and perfectly integrated with host muscles, thus confirming the suitability of P(BCE-co-TECE) scaffolds as substrates for skeletal muscle tissue engineering. ; This work has been supported by FWO (#G060612N, #G0A8813N, and #G088715N), CARIPLO Foundation #2015_0634, Opening the Future Campaign #EJJ-OPTFUT-02010, Excellentiefinanciering KULStem Cells #ETH-C1900-PF grant, Rondoufondsvoor Duchenne Onderzoek and the Belgian Agency for Science Policy IUAPVII-07 DevRepair (Belspo) network to M.S. EB was supported by FWO Post-Doctoral Fellowship (12D2813N) and an FWO grant (1525315N). We would like to acknowledge the support from the FP7 COST Action MP1206 “Electrospun Nanofibres for Bioinspired Composite Materials and Innovative Industrial Applications”. ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 19 (10) (Art N° 3212); http://hdl.handle.net/1942/33214; 10; 19; WOS:000448951000367; http://www.mdpi.com/1422-0067/19/10/3212 |
| DOI: | 10.3390/ijms19103212 |
| الاتاحة: | http://hdl.handle.net/1942/33214 https://doi.org/10.3390/ijms19103212 http://www.mdpi.com/1422-0067/19/10/3212 |
| رقم الانضمام: | edsbas.49133EC1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms19103212 |
|---|