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Silver Nanostar-Based SERS for the Discrimination of Clinically Relevant Acinetobacter baumannii and Klebsiella pneumoniae Species and Clones

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العنوان: Silver Nanostar-Based SERS for the Discrimination of Clinically Relevant Acinetobacter baumannii and Klebsiella pneumoniae Species and Clones
المؤلفون: de Almeida, Miguel Peixoto, Rodrigues, Carla, Novais, Ângela, Grosso, Filipa, Leopold, Nicolae, Peixe, Luísa, Franco, Ricardo, Pereira, Eulália
المساهمون: UCIBIO - Applied Molecular Biosciences Unit, DQ - Departamento de Química
سنة النشر: 2023
المجموعة: Repositório da Universidade Nova de Lisboa (UNL)
مصطلحات موضوعية: bacterial differentiation, chemometrics, clones, critical pathogens, SERS, silver nanostars, surface-enhanced Raman spectroscopy, vibrational spectroscopy, Analytical Chemistry, Biotechnology, Biomedical Engineering, Instrumentation, Engineering (miscellaneous), Clinical Biochemistry, SDG 3 - Good Health and Well-being
الوصف: And project HEALTH-UNORTE I&D&I NORTE-01-0145-FEDER-000039 cofinanced by European Regional Development Fund (ERDF), through the NORTE 2020 (Programa Operacional Regional do Norte 2014/2020). AN was supported by Portuguese funds through FCT in the context of the CEEC program (2021.02252.CEECIND/CP1662/CT0009). FG was supported by Portuguese funds through FCT in the context of the transitional norm (DL57/2016/CP1346/CT0034). NL acknowledges support from a grant of the Romanian Ministry of Research and Innovation, CCCDI-UEFISCDI, project number PN-III-P4-ID-PCE-2020-1292, within PNCDI III. Publisher Copyright: © 2023 by the authors. ; The development of rapid, reliable, and low-cost methods that enable discrimination among clinically relevant bacteria is crucial, with emphasis on those listed as WHO Global Priority 1 Critical Pathogens, such as carbapenem-resistant Acinetobacter baumannii and carbapenem-resistant or ESBL-producing Klebsiella pneumoniae. To address this problem, we developed and validated a protocol of surface-enhanced Raman spectroscopy (SERS) with silver nanostars for the discrimination of A. baumannii and K. pneumoniae species, and their globally disseminated and clinically relevant antibiotic resistant clones. Isolates were characterized by mixing bacterial colonies with silver nanostars, followed by deposition on filter paper for SERS spectrum acquisition. Spectral data were processed with unsupervised and supervised multivariate data analysis methods, including principal component analysis (PCA) and partial least-squares discriminant analysis (PLSDA), respectively. Our proposed SERS procedure using silver nanostars adsorbed to the bacteria, followed by multivariate data analysis, enabled differentiation between and within species. This pilot study demonstrates the potential of SERS for the rapid discrimination of clinically relevant A. baumannii and K. pneumoniae species and clones, displaying several advantages such as the ease of silver nanostars synthesis and the possible use of a ...
نوع الوثيقة: article in journal/newspaper
اللغة: English
تدمد: 2079-6374
Relation: Funding Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT; info:eu-repo/grantAgreement/FCT/CEEC IND4ed/2021.02252.CEECIND%2FCP1662%2FCT0009/PT; PURE: 72486060; PURE UUID: 7bf6f7a2-016f-4335-b8cc-e12a38c9a4bc; Scopus: 85148963566; WOS: 000938915500001; PubMed: 36831915; PubMedCentral: PMC9953856; http://hdl.handle.net/10362/158341; https://doi.org/10.3390/bios13020149
DOI: 10.3390/bios13020149
الاتاحة: http://hdl.handle.net/10362/158341
https://doi.org/10.3390/bios13020149
Rights: openAccess
رقم الانضمام: edsbas.48ABBE64
قاعدة البيانات: BASE
الوصف
تدمد:20796374
DOI:10.3390/bios13020149