التفاصيل البيبلوغرافية
| العنوان: |
Bacteria-Based Live Vehicle for In Vivo Bioluminescence Imaging |
| المؤلفون: |
Tianyu Jiang (1376670), Xingye Yang (7066124), Geng Li (312002), Xiaohan Zhao (8603151), Tao Sun (91038), Rolf Müller (1426348), Hailong Wang (21678), Minyong Li (69472), Youming Zhang (565208) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Medicine, Microbiology, Molecular Biology, Biotechnology, Immunology, Cancer, Science Policy, Infectious Diseases, Computational Biology, Space Science, Biological Sciences not elsewhere classified, Information Systems not elsewhere classified, based imageable vehicle, >, thus providing, genomically integrated fluc, based live vehicle, assisted cancer therapy, ecn ), one, recombinant ecn strain, cancer therapy, lre ), vivo <, vitro <, targeting system, stable bioluminescence, shifted bioluminescence, lre cassette |
| الوصف: |
The anticancer therapy strategy mediated by tumor-targeting bacteria needs better visualization tools for imaging and monitoring bacteria in vivo . The probiotic strain Escherichia coli Nissle 1917 (EcN), one of the tumor-targeting bacteria, leads to the potential application for cancer therapy. Here, we report the development and application of a live, EcN-based imageable vehicle for noninvasive in vivo bioluminescence imaging in live mice. Firefly luciferase (Fluc) and luciferin-regenerating enzyme (LRE), an enzyme that contributes to stable bioluminescence, were functionally coexpressed in EcN. The recombinant EcN strain expressing the genomically integrated Fluc-LRE cassette was demonstrated to be a valuable tool for generating robust, continuous, and red-shifted bioluminescence for bacterial tracking in vitro and in vivo , thus providing an optical tumor-targeting system for the in vivo study of bacteria-assisted cancer therapy. Additionally, in vivo imaging of the recombinant EcN strain in the mouse intestinal tract indicated the potential of this strain to be used as a tool in the study of gut. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Bacteria-Based_Live_Vehicle_for_i_In_Vivo_i_Bioluminescence_Imaging/17026654 |
| DOI: |
10.1021/acs.analchem.1c03568.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.analchem.1c03568.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.488BFC9A |
| قاعدة البيانات: |
BASE |